CAJ | 학술논문

建立了中国对虾虾青素液相色谱检测方法，采用 Develosil C30色谱柱为分析柱，甲醇∶乙腈（80∶20，v ／v）为流动相，采用二极管阵列检测器（DAD）分析，外标法定量，同时对虾头和虾壳虾青素的超声波提取工艺进行了优化研究。结果表明，中国对虾虾青素主要是以游离形式存在，虾青素在1～32μg／mL 范围内线性良好，相关系数为0．9993。通过响应面试验对超声波提取虾青素的工艺进行了优化，得到超声波辅助提取虾青素的最佳条件为：液固比430 mL／g、超声波功率450 W、超声时间8．5 min，虾青素得率的预测值为14．02μg／g，在优化条件下，虾青素得率测定值为13．89μg／g，相对误差为0．9％，与预测结果基本相符。
건립료중국대하하청소액상색보검측방법，채용 Develosil C30색보주위분석주，갑순∶을정（80∶20，v ／v）위류동상，채용이겁관진렬검측기（DAD）분석，외표법정량，동시대하두화하각하청소적초성파제취공예진행료우화연구。결과표명，중국대하하청소주요시이유리형식존재，하청소재1～32μg／mL 범위내선성량호，상관계수위0．9993。통과향응면시험대초성파제취하청소적공예진행료우화，득도초성파보조제취하청소적최가조건위：액고비430 mL／g、초성파공솔450 W、초성시간8．5 min，하청소득솔적예측치위14．02μg／g，재우화조건하，하청소득솔측정치위13．89μg／g，상대오차위0．9％，여예측결과기본상부。
Abstrat:A gradient high performance liquid chromatography (HPLC)was developed for the detection of astaxanthin from Fenneropenaeus chinensis.Develosil C30 column was used as analytical column,methanol-ace-tonitrile (80∶20,v /v)as mobile phase,analyzed by diode array detector (DAD),quantified by external stand-ard method;and the extracting technique assisted by ultrasound assisted extraction method of astaxanthin from Fenneropenaeus chinensis shell and head was optimized.The results showed that free astaxanthin was the mainly form exists in Fenneropenaeus chinensis shell and head,the linear range of astaxanthin was 1 ~32 μg/mL (r2 =0.9993).The extraction technique of ultrasound assisted Fenneropenaeus chinensis astaxanthin extraction was optimized by response surface methodology,the optimal condition for extraction technique was that L/S 430 mL/g,ultrasonic power 450 W,ultrasonic time 8.5 min,under the optimal condition,the predicted astaxanthin yield was 14.02 μg/g,the measured value was 13.89 μg/g,predictive value of relative error was 0.9%,and the predicted results were basically consistent with the result of predicted.