色谱
色譜
색보
Chinese Journal of Chromatography
2015年
11期
1140-1146
,共7页
徐基伟%孙元社%唐涛%夏明珠%雷武%王风云%李彤
徐基偉%孫元社%唐濤%夏明珠%雷武%王風雲%李彤
서기위%손원사%당도%하명주%뢰무%왕풍운%리동
混合色谱%反相/离子交换混合固定相%反相/亲水混合固定相%亲水/离子交换混合固定相%两性离子交换混合固定相
混閤色譜%反相/離子交換混閤固定相%反相/親水混閤固定相%親水/離子交換混閤固定相%兩性離子交換混閤固定相
혼합색보%반상/리자교환혼합고정상%반상/친수혼합고정상%친수/리자교환혼합고정상%량성리자교환혼합고정상
mixed-mode chromatography%reversed-phase/ion-exchange mixed-mode stationa-ry phase%reversed-phase/hydrophilic mixed-mode stationary phase%hydrophilic/ion-exchange mixed-mode stationary phase%zwitterion exchange mixed-mode stationary phase
随着科学技术的发展,人们需要分离分析的样品越来越复杂,尤其是多肽、蛋白质类生物样品的复杂性使得单一模式色谱难以满足分离分析的要求。混合模式色谱因其独特的分离性能,可以在一次分离中获得与多维色谱相当的分离效果,而且可以避免多维色谱系统结构复杂、流动相兼容性差、分析时间长等问题,成为近年来的研究热点之一。混合模式色谱的研究重点是色谱固定相的设计与开发。混合模式色谱固定相包括反相/离子交换混合固定相、反相/亲水混合固定相、亲水/离子交换混合固定相、两性离子交换混合固定相及三相混合固定相。本文综述了近年来混合模式色谱填料的研究及应用进展,并对混合模式色谱及固定相的发展前景进行了展望。
隨著科學技術的髮展,人們需要分離分析的樣品越來越複雜,尤其是多肽、蛋白質類生物樣品的複雜性使得單一模式色譜難以滿足分離分析的要求。混閤模式色譜因其獨特的分離性能,可以在一次分離中穫得與多維色譜相噹的分離效果,而且可以避免多維色譜繫統結構複雜、流動相兼容性差、分析時間長等問題,成為近年來的研究熱點之一。混閤模式色譜的研究重點是色譜固定相的設計與開髮。混閤模式色譜固定相包括反相/離子交換混閤固定相、反相/親水混閤固定相、親水/離子交換混閤固定相、兩性離子交換混閤固定相及三相混閤固定相。本文綜述瞭近年來混閤模式色譜填料的研究及應用進展,併對混閤模式色譜及固定相的髮展前景進行瞭展望。
수착과학기술적발전,인문수요분리분석적양품월래월복잡,우기시다태、단백질류생물양품적복잡성사득단일모식색보난이만족분리분석적요구。혼합모식색보인기독특적분리성능,가이재일차분리중획득여다유색보상당적분리효과,이차가이피면다유색보계통결구복잡、류동상겸용성차、분석시간장등문제,성위근년래적연구열점지일。혼합모식색보적연구중점시색보고정상적설계여개발。혼합모식색보고정상포괄반상/리자교환혼합고정상、반상/친수혼합고정상、친수/리자교환혼합고정상、량성리자교환혼합고정상급삼상혼합고정상。본문종술료근년래혼합모식색보전료적연구급응용진전,병대혼합모식색보급고정상적발전전경진행료전망。
With the development of science and technology,more and more complex samples like peptides and proteins need to be separated. It is difficult to separate them by single mode chromatography. Due to the unique separation character of mixed-mode chromatography,the same separation performance can be obtained in mixed-mode chromatography in one separation as multidimensional chromatography. And some disadvantages of multidimensional chromatog-raphy can be avoided in mixed-mode chromatography,such as the complexity of the system, the poor compatibility of mobile phases and the long analytical time. More and more attention is devoted to the mixed-mode chromatography in recent years. The focus on the mixed-mode chromatography is to design new structures of mixed-mode stationary phase. At present, mixed-mode stationary phases included reversed-phase/ion-exchange,reversed-phase/hydro-philic,hydrophilic/ion-exchange,zwitterionic and trimode mixed-mode stationary phases. The kinds of mixed-mode stationary phases are summarized and their features and applications in different fields are discussed in this review.