CAJ | 학술논문

固定化酶反应器作为蛋白质组学研究中“bottom-up”策略重要的组件，具有酶解快速、酶解效率高、酶稳定性和活性高、简单易操作、能够与多种检测方式联用等优点，对于发展高效快速的蛋白质组学分析方法具有重要意义。本文就固定化酶反应器的制备方法及其在蛋白质组学中的应用做简单的概述，着重介绍酶的固定方法、固定化酶的载体、用于固定的酶的种类。近几年固定化酶反应器的研究集中于提高固酶量、保持酶活性、增加酶解效率、减小非特异性吸附等方面。研究结果表明，采用纳米材料、整体材料等新型载体，提高载体亲水性，采用多酶同时酶解等方法能够有效改善固定化酶反应器的性能，提高蛋白质的鉴定效率。
고정화매반응기작위단백질조학연구중“bottom-up”책략중요적조건，구유매해쾌속、매해효솔고、매은정성화활성고、간단역조작、능구여다충검측방식련용등우점，대우발전고효쾌속적단백질조학분석방법구유중요의의。본문취고정화매반응기적제비방법급기재단백질조학중적응용주간단적개술，착중개소매적고정방법、고정화매적재체、용우고정적매적충류。근궤년고정화매반응기적연구집중우제고고매량、보지매활성、증가매해효솔、감소비특이성흡부등방면。연구결과표명，채용납미재료、정체재료등신형재체，제고재체친수성，채용다매동시매해등방법능구유효개선고정화매반응기적성능，제고단백질적감정효솔。
As an important part in“bottom-up”strategy of proteomics,immobilized enzyme reactors have great significance in the development of fast and more efficient protein analytical method,owing to its advantages of high speed and enzymatic efficiency,good stability and activity,easy operation,and the possibility of hyphenating with multiple detection instruments. In this paper,the preparation methods of immobilized enzyme reactors and their applications in proteomic investigation are introduced,focusing on the nature of enzymes,the immobilization methods and the carrier materials used for immobilizing enzyme. In recent years,the investiga-tions are focused on increasing the immobilization amounts of enzyme,keeping enzymatic activity,improving enzymatic efficiency and decreasing nonspecific adsorption. The investiga-tion results showed that by using novel carriers such as nanomaterial and monolith,increasing of hydrophilicity of carrier and tandem hydrolysis with multiple enzymes can greatly improve the performance of immobilized enzyme reactors and increase protein identification efficiencies.