医学检验与临床
醫學檢驗與臨床
의학검험여림상
Medical Laboratory Science and Clinics
2015年
5期
20-22
,共3页
ITP%改良MAIPA法%抗GPIb抗体%抗GPIIbIIIa抗体
ITP%改良MAIPA法%抗GPIb抗體%抗GPIIbIIIa抗體
ITP%개량MAIPA법%항GPIb항체%항GPIIbIIIa항체
ITP%Modified MAIPA%GPIIb/IIIa and GPIb antibodies
目的：探讨二价阳离子络合剂的抗凝剂在改良MAIPA法检测ITP患者血小板特异性自身抗体中的影响。方法：选取159例ITP患者，采用改良MAIPA法分别检测EDTA-K2（络合二价阳离子）及肝素钠抗凝的ITP患者血浆，对两者检出率进行比较。结果：抗GPIb抗体检出情况：使用EDTA作为抗凝剂检出阳性88例（55.35%），肝素作为抗凝剂检出104例（65.41%），两者比较差异无统计学意义（χ2=3.35，P>0.05）；抗GPIIbIIIa抗体检出情况：使用EDTA作为抗凝剂检出阳性46例（28.93%），肝素作为抗凝剂检出91例（57.23%），两者比较差异有统计学意义（χ2=8.99，P<0.05）。结论：对于抗GPIIb/IIIa抗体，肝素作为抗凝剂的检出率要明显高于EDTA作为抗凝剂，而抗GPIb抗体，肝素作为抗凝剂与EDTA作为抗凝剂的检出率无明显差别。
目的：探討二價暘離子絡閤劑的抗凝劑在改良MAIPA法檢測ITP患者血小闆特異性自身抗體中的影響。方法：選取159例ITP患者，採用改良MAIPA法分彆檢測EDTA-K2（絡閤二價暘離子）及肝素鈉抗凝的ITP患者血漿，對兩者檢齣率進行比較。結果：抗GPIb抗體檢齣情況：使用EDTA作為抗凝劑檢齣暘性88例（55.35%），肝素作為抗凝劑檢齣104例（65.41%），兩者比較差異無統計學意義（χ2=3.35，P>0.05）；抗GPIIbIIIa抗體檢齣情況：使用EDTA作為抗凝劑檢齣暘性46例（28.93%），肝素作為抗凝劑檢齣91例（57.23%），兩者比較差異有統計學意義（χ2=8.99，P<0.05）。結論：對于抗GPIIb/IIIa抗體，肝素作為抗凝劑的檢齣率要明顯高于EDTA作為抗凝劑，而抗GPIb抗體，肝素作為抗凝劑與EDTA作為抗凝劑的檢齣率無明顯差彆。
목적：탐토이개양리자락합제적항응제재개량MAIPA법검측ITP환자혈소판특이성자신항체중적영향。방법：선취159례ITP환자，채용개량MAIPA법분별검측EDTA-K2（락합이개양리자）급간소납항응적ITP환자혈장，대량자검출솔진행비교。결과：항GPIb항체검출정황：사용EDTA작위항응제검출양성88례（55.35%），간소작위항응제검출104례（65.41%），량자비교차이무통계학의의（χ2=3.35，P>0.05）；항GPIIbIIIa항체검출정황：사용EDTA작위항응제검출양성46례（28.93%），간소작위항응제검출91례（57.23%），량자비교차이유통계학의의（χ2=8.99，P<0.05）。결론：대우항GPIIb/IIIa항체，간소작위항응제적검출솔요명현고우EDTA작위항응제，이항GPIb항체，간소작위항응제여EDTA작위항응제적검출솔무명현차별。
Objective:To investigate the effects of divalent cation complexing anticoagulants on the detection of platelet-specific autoantibodies by modified MAIPA assay in patients with ITP.Methods:We collected 159 cases of ITP patients, detected the plasmas anticoagulated by EDTA-K2 (complexed divalent cation) or heparin with the method of MAIPA respectively, and compared the detection rates of the two anticoagulants.Results: For the anti GPIb antibody: 88 cases were detected in EDTA group, while 104 cases were detected in heparin group, without statistically significant difference between the two groups (χ2=3.35,P>0.05); For the anti GPIIbIIIa antibody: 46 cases were detected in EDTA group, while 91 cases were detected in heparin group, with a statistically significant difference (χ2=8.99,P<0.05).Conclusions:For anti GPIIb/IIIa antibody, heparin group was significantly higher detected than that of EDTA group. For anti GPIb antibody, there was no obvious difference between them.
