CAJ | 학술논문

为探讨Cu2＋存在对橙皮素（ Hsp）清除自由基作用的影响．将Hsp与Cu2＋混合即生成不稳定配合物（Hsp－Cu），利用UV－Vis光谱测定Hsp及Hsp－Cu对1，1－二苯基－2－三硝基苯肼自由基（ DPPH·）、羟基自由基（· OH）、超氧阴离子自由基（ O2－·）的清除作用．在实验条件下Hsp－Cu对DPPH·及· OH有一定的清除作用，但清除能力不如Hsp．Hsp－Cu不能有效清除O2－·，且在邻苯三酚自氧化过程中表现出促氧化作用．表明Hsp作为抗氧化剂使用时，应避免与Cu2＋接触，以免影响其抗氧化能力．
위탐토Cu2＋존재대등피소（ Hsp）청제자유기작용적영향．장Hsp여Cu2＋혼합즉생성불은정배합물（Hsp－Cu），이용UV－Vis광보측정Hsp급Hsp－Cu대1，1－이분기－2－삼초기분정자유기（ DPPH·）、간기자유기（· OH）、초양음리자자유기（ O2－·）적청제작용．재실험조건하Hsp－Cu대DPPH·급· OH유일정적청제작용，단청제능력불여Hsp．Hsp－Cu불능유효청제O2－·，차재린분삼분자양화과정중표현출촉양화작용．표명Hsp작위항양화제사용시，응피면여Cu2＋접촉，이면영향기항양화능력．
To study the affection of Copper ( II ) ions on radical scavenging of hesperetin . Unstable hesperetin -copper ( II ) complex was generated when hesperetin was mixed with copper ( II ) chloride dihydrate .By the contrast tests on the scavenging reaction to DPPH radical, hydroxyl radical (· OH), and superoxide free radical (O2 -· ) of hesperetin -copper ( II) complex and hesperetin , proves that the scavenging activity of DPPH · and · OH of hesperetin-copper ( II) complex was weaker than that of hesperetin .The hesperetin-copper (II) complex can’t scavenge O2 -· showing accelerated oxidation in the process of pyrogallol autoxidation .Hesperetin was used as an antioxidant , cannot coexist with Cu 2+, so as not to affect the antioxidant capacity of hesperetin .