CAJ | 학술논문

研究芪丑汤拆方对肾间质纤维化大鼠肾组织内质网应激分子伴侣作用的机理．通过单侧输尿管梗阻建立大鼠肾间质纤维化的动物模型．将大鼠随机分为假手术组、模型组、依那普利组、芪丑汤全方组、温补脾肾组、泄浊化瘀组．分别于造模后第7天、第14天处死大鼠，收集大鼠血清测定肌酐（ Scr）、尿素氮（ BUN）；摘取左侧肾脏，称肾脏重量比较分析，观察肾脏水肿程度；采用HE染色评定肾小管损伤指数，及肾间质纤维化程度；采用Western Blot 免疫印迹法检测各实验组内质网分子伴侣GRP78、GRP94蛋白表达水平．结果表明，术后第7、14天，各治疗组与模型组比较，Scr、BUN的水平均明显降低（P＜0．01，P＜0．05）；第7天芪丑汤全方组与依那普利组比较Scr水平有差异（P＜0．05）；第14天芪丑汤全方组与依那普利组比较，降低Scr、BUN作用明显（P＜0．05）；术后第7、14天，除14天的温补脾肾组外各治疗组与模型组相比，均能减小肾重（P＜0．01，P＜0．05）；芪丑汤全方组与依那普利组比较能减轻肾重（P＜0．05）；术后第7天芪丑汤全方组与模型组相比，能显著减轻肾小管损伤（P＜0．05）；14天芪丑汤全方组与依那普利组比较，肾小管损伤作用有差异（P＜0．05）；术后第14天，芪丑汤全方组和泄浊化瘀组与模型组比较GRP78、GRP94的蛋白表达有显著差异（ P＜0．01），与依那普利组比较，芪丑汤全方组能显著抑制GRP78、GRP94的表达（P＜0．01）．芪丑汤全方组能够保护单侧输尿管梗阻大鼠的肾功能；减轻肾脏肾盂积水；降低肾小管损伤指数；减少GRP78、GRP94的蛋白表达从而干扰内质网应激过程介导的细胞凋亡通路，阻碍细胞凋亡沉积引起的肾间质纤维化．各治疗组14天的作用效果优于7天，泄浊化瘀组优于温补脾肾组．研究芪醜湯拆方對腎間質纖維化大鼠腎組織內質網應激分子伴侶作用的機理．通過單側輸尿管梗阻建立大鼠腎間質纖維化的動物模型．將大鼠隨機分為假手術組、模型組、依那普利組、芪醜湯全方組、溫補脾腎組、洩濁化瘀組．分彆于造模後第7天、第14天處死大鼠，收集大鼠血清測定肌酐（ Scr）、尿素氮（ BUN）；摘取左側腎髒，稱腎髒重量比較分析，觀察腎髒水腫程度；採用HE染色評定腎小管損傷指數，及腎間質纖維化程度；採用Western Blot 免疫印跡法檢測各實驗組內質網分子伴侶GRP78、GRP94蛋白錶達水平．結果錶明，術後第7、14天，各治療組與模型組比較，Scr、BUN的水平均明顯降低（P＜0．01，P＜0．05）；第7天芪醜湯全方組與依那普利組比較Scr水平有差異（P＜0．05）；第14天芪醜湯全方組與依那普利組比較，降低Scr、BUN作用明顯（P＜0．05）；術後第7、14天，除14天的溫補脾腎組外各治療組與模型組相比，均能減小腎重（P＜0．01，P＜0．05）；芪醜湯全方組與依那普利組比較能減輕腎重（P＜0．05）；術後第7天芪醜湯全方組與模型組相比，能顯著減輕腎小管損傷（P＜0．05）；14天芪醜湯全方組與依那普利組比較，腎小管損傷作用有差異（P＜0．05）；術後第14天，芪醜湯全方組和洩濁化瘀組與模型組比較GRP78、GRP94的蛋白錶達有顯著差異（ P＜0．01），與依那普利組比較，芪醜湯全方組能顯著抑製GRP78、GRP94的錶達（P＜0．01）．芪醜湯全方組能夠保護單側輸尿管梗阻大鼠的腎功能；減輕腎髒腎盂積水；降低腎小管損傷指數；減少GRP78、GRP94的蛋白錶達從而榦擾內質網應激過程介導的細胞凋亡通路，阻礙細胞凋亡沉積引起的腎間質纖維化．各治療組14天的作用效果優于7天，洩濁化瘀組優于溫補脾腎組．
연구기축탕탁방대신간질섬유화대서신조직내질망응격분자반려작용적궤리．통과단측수뇨관경조건립대서신간질섬유화적동물모형．장대서수궤분위가수술조、모형조、의나보리조、기축탕전방조、온보비신조、설탁화어조．분별우조모후제7천、제14천처사대서，수집대서혈청측정기항（ Scr）、뇨소담（ BUN）；적취좌측신장，칭신장중량비교분석，관찰신장수종정도；채용HE염색평정신소관손상지수，급신간질섬유화정도；채용Western Blot 면역인적법검측각실험조내질망분자반려GRP78、GRP94단백표체수평．결과표명，술후제7、14천，각치료조여모형조비교，Scr、BUN적수평균명현강저（P＜0．01，P＜0．05）；제7천기축탕전방조여의나보리조비교Scr수평유차이（P＜0．05）；제14천기축탕전방조여의나보리조비교，강저Scr、BUN작용명현（P＜0．05）；술후제7、14천，제14천적온보비신조외각치료조여모형조상비，균능감소신중（P＜0．01，P＜0．05）；기축탕전방조여의나보리조비교능감경신중（P＜0．05）；술후제7천기축탕전방조여모형조상비，능현저감경신소관손상（P＜0．05）；14천기축탕전방조여의나보리조비교，신소관손상작용유차이（P＜0．05）；술후제14천，기축탕전방조화설탁화어조여모형조비교GRP78、GRP94적단백표체유현저차이（ P＜0．01），여의나보리조비교，기축탕전방조능현저억제GRP78、GRP94적표체（P＜0．01）．기축탕전방조능구보호단측수뇨관경조대서적신공능；감경신장신우적수；강저신소관손상지수；감소GRP78、GRP94적단백표체종이간우내질망응격과정개도적세포조망통로，조애세포조망침적인기적신간질섬유화．각치료조14천적작용효과우우7천，설탁화어조우우온보비신조．
To investigate its mechanism of Qichou Tang decomposed group on renal tissue en-doplasmic reticulum stress chaperone of rats with renal interstitial fibrosis .Unilateral ligation of ureter was carried out to establish the rat model of renal interstitial fibrosis .The rats were randomly divided into sham operation group , model group , enalapril group , Qichou Tang group, warming spleen group , Xiezhuo Huayu group .On the 7th and 14th day after model-ing, the rats were killed, respectively.The serum was collected for measuring of creatinine ( Scr) and blood urea nitrogen ( BUN);the left kidneys which were taken out were weighed for analysis and comparison , renal edema was observed; the assessment of tubular damage index and the degree of renal interstitial fibrosis were measured by HE staining; protein ex-pression levels of endoplasmic reticulum chaperones GRP 78, GRP94 in each experimental group were measured by Western blotting .Results indicated that Scr , BUN levels were sig-nificantly lower in treatment group and model group on the 7th and 14th day (P <0.01, P<0.05);Scr level was a significant difference in Qichou Tang group and enalapril group on 7th day (P <0.05);it is significant that Scr, BUN levels were obvious reduced in Qichou Tang group and enalapril group on 14th day ( P <0.05); in addition to Warming spleen group on the 14th day, kidney weight was reduced in all treatment group after 7th and 14th day (P <0.01, P <0.05) ;kidney weight was significantly reduced in Qichou Tang group and enalapril group (P <0.05);Qichou Tang group could more significantly alleviate renal tubular injury than the model group on 7th day(P <0.05); the difference in renal tubular injury was observed in Qichou Tang group and enalapril group on 14th day ( P <0.05);there was significant difference in protein expressions of GRP 78 , GRP94 in Qichou Tang group and Xiezhuo Huayu group compared with model group on 14th day(P <0.01), Qi-chou Tang group could more significantly inhibit protein expression of GRP 78, GRP94 than the enalapril group (P <0.01).Qichou Tang group can protect kidney function of rats with unilateral ureteral obstruction;alleviate kidney hydronephrosis;reduce renal tubular damage index; reduce the protein expressions of GRP78, GRP94, thus interference apoptotic path-way of ER stress-mediated process; impede renal interstitial fibrosis induced by apoptosis deposition.The role of each treatment group on 14th day was better than that on 7th day, Xiezhuo Huayu group was better than warming spleen group .