徐州医学院学报
徐州醫學院學報
서주의학원학보
Acta Academiae Medicinae Xuzhou
2015年
9期
571-576
,共6页
乳腺癌%信号通路%表皮生长因子受体%磷酸化%预后
乳腺癌%信號通路%錶皮生長因子受體%燐痠化%預後
유선암%신호통로%표피생장인자수체%린산화%예후
breast cancer%signaling pathway%epidermal growth factor receptor%phosphorylation%prognosis
目的：探讨表皮生长因子受体（ EGFR）的磷酸化水平与乳腺癌细胞生物学行为及患者术后生存期的关系。方法采用双染免疫组化检测60例乳腺癌组织中EGFR和磷酸化EGFR（ p-EGFR）的表达，分析两者与乳腺癌生物学行为、术后无瘤生存期及总生存期的关系。结果60例乳腺癌标本EGFR阳性率81．67％（49／60），p-EGFR阳性率60．0％（36／60）。 EGFR阳性率与分化程度密切相关，而p-EGFR则与分化程度、淋巴结转移、临床分期密切相关，在低分化、有淋巴结转移、进展晚期的乳腺癌中表达明显升高。 Kaplan-Meier生存曲线及log-rank分析发现，EGFR与乳腺癌患者术后总生存期（ OS）和无瘤生存期（ DFS）无关，而p-EGFR表达水平与乳腺癌患者无瘤生存期（ DFS）显著相关，p-EGFR高表达组患者DFS比阴性组乳腺癌患者缩短。结论乳腺癌组织EGFR蛋白磷酸化激活是影响乳腺癌患者术后生存期的重要因素。 EGFR被激活后的磷酸化水平能更好地反映乳腺癌的生物学行为，并对患者的预后判断有一定帮助。
目的：探討錶皮生長因子受體（ EGFR）的燐痠化水平與乳腺癌細胞生物學行為及患者術後生存期的關繫。方法採用雙染免疫組化檢測60例乳腺癌組織中EGFR和燐痠化EGFR（ p-EGFR）的錶達，分析兩者與乳腺癌生物學行為、術後無瘤生存期及總生存期的關繫。結果60例乳腺癌標本EGFR暘性率81．67％（49／60），p-EGFR暘性率60．0％（36／60）。 EGFR暘性率與分化程度密切相關，而p-EGFR則與分化程度、淋巴結轉移、臨床分期密切相關，在低分化、有淋巴結轉移、進展晚期的乳腺癌中錶達明顯升高。 Kaplan-Meier生存麯線及log-rank分析髮現，EGFR與乳腺癌患者術後總生存期（ OS）和無瘤生存期（ DFS）無關，而p-EGFR錶達水平與乳腺癌患者無瘤生存期（ DFS）顯著相關，p-EGFR高錶達組患者DFS比陰性組乳腺癌患者縮短。結論乳腺癌組織EGFR蛋白燐痠化激活是影響乳腺癌患者術後生存期的重要因素。 EGFR被激活後的燐痠化水平能更好地反映乳腺癌的生物學行為，併對患者的預後判斷有一定幫助。
목적：탐토표피생장인자수체（ EGFR）적린산화수평여유선암세포생물학행위급환자술후생존기적관계。방법채용쌍염면역조화검측60례유선암조직중EGFR화린산화EGFR（ p-EGFR）적표체，분석량자여유선암생물학행위、술후무류생존기급총생존기적관계。결과60례유선암표본EGFR양성솔81．67％（49／60），p-EGFR양성솔60．0％（36／60）。 EGFR양성솔여분화정도밀절상관，이p-EGFR칙여분화정도、림파결전이、림상분기밀절상관，재저분화、유림파결전이、진전만기적유선암중표체명현승고。 Kaplan-Meier생존곡선급log-rank분석발현，EGFR여유선암환자술후총생존기（ OS）화무류생존기（ DFS）무관，이p-EGFR표체수평여유선암환자무류생존기（ DFS）현저상관，p-EGFR고표체조환자DFS비음성조유선암환자축단。결론유선암조직EGFR단백린산화격활시영향유선암환자술후생존기적중요인소。 EGFR피격활후적린산화수평능경호지반영유선암적생물학행위，병대환자적예후판단유일정방조。
Objective To explore the relationship between phosphorylated levels of epidermal growth factor receptor ( EGFR) and the biological behaviors of breast cancer and the postoperative survival of cancer patients.Methods The levels of total EGFR and phosphorylated EGFR ( p-EGFR) were detected in sixty breast cancer tissues by double immu-nohistochemical staining.Then, their relationship with the biological behaviors of breast cancer, the postoperative disease-free survival ( DFS) and overall survival in cancer patients were analyzed.Results These cancer specimens showed a positive rate of 81.67%(49/60) for EGFR and 60.0%(36/60) for p-EGFR.The positive rate of EGFR was related with the differentiation of cancer cells.In contrast, the positive rate of p-EGFR was associated with the differentiation of cancer cells, lymph node metastasis and clinical phases.The amount of p-EGFR was remarkably increased in poorly differentiated, advanced cancer with lymph node metastasis.According to the Kaplan-Meier curve and log-rank analy-sis, the level of EGFR was not related with DFS and overall survival of cancer patients, while the quantity of p-EGFR was closely associated with DFS of cancer patients.The patients presenting a high level of p-EGFR showed a shorter DFS than those with negative p-EGFR expression.Conclusion The phosphorylation of EGFR is an important factor as-sociated with postoperative survival in breast cancer patients.The level of phosphorylated EGFR can reflect the biological behaviors of breast cancer, which is helpful for assessing the prognosis of patients.
