哈尔滨商业大学学报（自然科学版）
哈爾濱商業大學學報（自然科學版）
합이빈상업대학학보（자연과학판）
Journal of Harbin University of Commerce (Natural Sciences Edition)
2015年
5期
540-542
,共3页
刘菲%李明%盛明明%阎雪莹
劉菲%李明%盛明明%閻雪瑩
류비%리명%성명명%염설형
木犀草素%脂质体%乙醇注入法%正交实验
木犀草素%脂質體%乙醇註入法%正交實驗
목서초소%지질체%을순주입법%정교실험
luteolin%liposome%ethanol injection%orthogonal test
探讨乙醇注入法制备木犀草素脂质体的制备工艺．采用乙醇注入法制备木犀草素脂质体，微柱离心和HPLC法测定包封率，激光粒度仪测定粒径．运用正交实验设计L9（34）优选最佳处方为：磷脂600 mg，药脂比1∶7，类酯比8∶1，有机相水相比1∶1，包封率79．3％，形态，稳定性良好．注入法制备木犀草素脂质体工艺简单，制得剂型稳定，方法可行．
探討乙醇註入法製備木犀草素脂質體的製備工藝．採用乙醇註入法製備木犀草素脂質體，微柱離心和HPLC法測定包封率，激光粒度儀測定粒徑．運用正交實驗設計L9（34）優選最佳處方為：燐脂600 mg，藥脂比1∶7，類酯比8∶1，有機相水相比1∶1，包封率79．3％，形態，穩定性良好．註入法製備木犀草素脂質體工藝簡單，製得劑型穩定，方法可行．
탐토을순주입법제비목서초소지질체적제비공예．채용을순주입법제비목서초소지질체，미주리심화HPLC법측정포봉솔，격광립도의측정립경．운용정교실험설계L9（34）우선최가처방위：린지600 mg，약지비1∶7，류지비8∶1，유궤상수상비1∶1，포봉솔79．3％，형태，은정성량호．주입법제비목서초소지질체공예간단，제득제형은정，방법가행．
To investigate the preparation of ethanol injection of luteolin liposome , the lipo-some was prepared by ethanol injection .Entrapment rate was measured by microcolumn off-center and HPLC .The particle size of luteolin liposome was measured by using the laser par-ticle size analyzer.Orthognal test L9(34)was applied to choose the best method, and the re-sult was as follows:PC (600 mg), PC∶Cho (6∶1), cho∶PC (1∶9), ethanol∶PBS (1∶1), entrapment rate of 79.3%.The shape and stability were good .The method of ethanol injec-tion of luteolin liposome was easy , and the dosage was stable .So this method could be ap-plied in luteolin liposome preparation .
