CAJ | 학술논문

采用RP－HPLC法同时测定北五味子中6种木脂素类成分．色谱柱为Agilent ZORBAX SB－C18（5μm，4．6×150 mm），流动相为甲醇－水溶液梯度洗脱，检测波长为254 nm，柱温为25℃．结果表明：五味子甲素在40．4～600．0μg／mL（r＝0．9998）、五味子乙素在42．0～420．0μg／mL（r＝0．9999）、五味子丙素在15．8～118．8μg／mL（r＝0．9995）、五味子醇甲在10．0～160．0μg／mL（r＝0．9999）、五味子醇乙在15．5～388．0μg／mL（r＝0．9995）、五味子酯甲在16．3～408．0μg／mL（r＝0．9999）范围内峰面积与对照品质量浓度呈良好的线性关系（n＝6）．6种成分的加样回收率均高于98％，RSD值小于3％本方法简便，准确，快速，重现性好，可用于北五味子中有效成分的定量分析．
채용RP－HPLC법동시측정북오미자중6충목지소류성분．색보주위Agilent ZORBAX SB－C18（5μm，4．6×150 mm），류동상위갑순－수용액제도세탈，검측파장위254 nm，주온위25℃．결과표명：오미자갑소재40．4～600．0μg／mL（r＝0．9998）、오미자을소재42．0～420．0μg／mL（r＝0．9999）、오미자병소재15．8～118．8μg／mL（r＝0．9995）、오미자순갑재10．0～160．0μg／mL（r＝0．9999）、오미자순을재15．5～388．0μg／mL（r＝0．9995）、오미자지갑재16．3～408．0μg／mL（r＝0．9999）범위내봉면적여대조품질량농도정량호적선성관계（n＝6）．6충성분적가양회수솔균고우98％，RSD치소우3％본방법간편，준학，쾌속，중현성호，가용우북오미자중유효성분적정량분석．
In this paper , RP-HPLC to simultaneously determine 6 kinds of lignans in fruc-tus schisandrae chinensis was adopted .The column was Agilent ZORBAX SB -C18 (5 μm, 4.6 ×150 mm).The mobile phase was consisted of methanol -water solution(gradient elu-tion).The detect wavelength was set at 254 nm, and the column temperature was 25 ℃. Results indicated that deoxyschizandrin at the range of 40.4~600.0μg/mL (r=0.999 8),γ-Schisandrin at the range of 42.0~420.0 μg/mL (r=0.999 9), Schisandrin C at the range of 15.8~118.8μg/mL (r=0.999 5), Schisandrin at the range of 10.0~160.0μg/mL (r =0.999 9), Schisandrol at the range of 15.5 ~388.0 μg/mL ( r =0.999 5) Schisantherin A at the range of 16.3~408.0 μg/mL (r=0.999 9) have good linear rela-tionship between peak area and concentration of reference substance ( n=6 ) .The sample recovery rate of 6 kinds of ingredients was higher than 98%, and the RSD value was less than 3%.This method was simple , accurate , celerity and good reproducibility , and can be used for quantitative analysis of effective ingredients of fructus schisandrae chinensis.