CAJ | 학술논문

目的：探讨糖调节受损大鼠海马神经细胞凋亡与学习记忆能力的关系。方法用高脂高糖饲料饲养法建立糖调节受损大鼠模型；用Morris水迷宫法检测学习记忆能力；用TUNEL法检测海马神经细胞凋亡数目；用免疫组化和原位杂交技术检测海马神经细胞Bcl-2／Bax和Bcl-2 mRNA／Bax mRNA的表达。结果与NGT组相比，IGR组学习记忆能力下降（P＜0.05），海马神经细胞凋亡数增高（P＜0.05），Bcl-2、Bcl-2 mRNA表达减少（P＜0.05），Bax与Bax mRNA表达增加（ P＜0.05）。 IGR组学习、记忆能力与Bcl-2阳性表达呈正相关（ P＜0.05），与Bax阳性表达呈负相关（ P＜0.05）。结论糖调节受损与大鼠学习记忆能力下降有相关性，海马神经细胞凋亡可能是其重要机制之一。
목적：탐토당조절수손대서해마신경세포조망여학습기억능력적관계。방법용고지고당사료사양법건립당조절수손대서모형；용Morris수미궁법검측학습기억능력；용TUNEL법검측해마신경세포조망수목；용면역조화화원위잡교기술검측해마신경세포Bcl-2／Bax화Bcl-2 mRNA／Bax mRNA적표체。결과여NGT조상비，IGR조학습기억능력하강（P＜0.05），해마신경세포조망수증고（P＜0.05），Bcl-2、Bcl-2 mRNA표체감소（P＜0.05），Bax여Bax mRNA표체증가（ P＜0.05）。 IGR조학습、기억능력여Bcl-2양성표체정정상관（ P＜0.05），여Bax양성표체정부상관（ P＜0.05）。결론당조절수손여대서학습기억능력하강유상관성，해마신경세포조망가능시기중요궤제지일。
Objective To identify the relationship between impaired glucose regulation of neuronal apoptosis in hip-pocampus and the ability of learning/memory in rats.Methods The model rats were made by high fat and sugar diet;The morris water maze was used to detect the learning and memory function;The TUNEL method was used to detect neuronal apoptosis in hippocampus .Expression of Bcl-2/Bax and Bcl-2 mRNA/Bax mRNA factor in hippo-campus neurons was detected with immunohistochemistry and in situ hybridization .Results Compared with NGT group, in IGR group the learning and memory ability were meaningfully decreased (P<0.05);the number of neuro-nal apoptosis in hippocampus was increased significantly ( P<0.05 ); the expression of Bcl-2 and Bcl-2 mRNA in hippocampus decreased significantly ( P<0.05);the expression of Bax and Bax mRNA in hippocampus increased sig-nificantly ( P<0.05 );The ability of learning and memory was positively correlated with expression of Bcl -2 ( P<0.05) and negatively correlated to the expression of Bax (P<0.05).Conclusions There is a relationship between impaired glucose regulation and the ability of learning and memory in rats , its mechanism may be potentially related to hippocampal neuronal apoptosis .