CAJ | 학술논문

目的：观察汉黄芩素对人γδT细胞杀伤胃癌SGC7901细胞的影响及机制。方法异戊烯焦磷酸（ IPP）法体外扩增人外周血γδT细胞，不同浓度的汉黄芩素作用于γδT细胞，四甲基偶氮唑蓝（ MTT）法检测汉黄芩素对γδT细胞生长的影响；流式细胞术（ FCM）检测汉黄芩素作用前后γδT细胞穿孔素、颗粒酶B、γ-干扰素（ INF-γ）、CD107a的表达；乳酸脱氢酶（ LDH）释放法检测汉黄芩素对γδT细胞杀伤胃癌SGC7901细胞活性的影响。结果 IPP作用10天，γδT细胞比例由2．51％增加到80．12％。与对照组比较，汉黄芩素在0．1～6．25 mg／L时对γδT细胞的生长有促进作用（P＜0．05）。经一定浓度的汉黄芩素诱导后γδT细胞的穿孔素、颗粒酶B、INF-γ、CD107a的表达显著高于对照组（P＜0．05）；对胃癌SGC7901细胞的杀伤活性也显著高于未诱导组（P＜0．05）。结论汉黄芩素在一定浓度范围内能够促进γδT细胞的增殖，并增强其对胃癌SGC7901细胞的杀伤能力，其机制可能与上调γδT细胞表面的穿孔素、颗粒酶B、INF-γ、CD107a的表达有关。
목적：관찰한황금소대인γδT세포살상위암SGC7901세포적영향급궤제。방법이무희초린산（ IPP）법체외확증인외주혈γδT세포，불동농도적한황금소작용우γδT세포，사갑기우담서람（ MTT）법검측한황금소대γδT세포생장적영향；류식세포술（ FCM）검측한황금소작용전후γδT세포천공소、과립매B、γ-간우소（ INF-γ）、CD107a적표체；유산탈경매（ LDH）석방법검측한황금소대γδT세포살상위암SGC7901세포활성적영향。결과 IPP작용10천，γδT세포비례유2．51％증가도80．12％。여대조조비교，한황금소재0．1～6．25 mg／L시대γδT세포적생장유촉진작용（P＜0．05）。경일정농도적한황금소유도후γδT세포적천공소、과립매B、INF-γ、CD107a적표체현저고우대조조（P＜0．05）；대위암SGC7901세포적살상활성야현저고우미유도조（P＜0．05）。결론한황금소재일정농도범위내능구촉진γδT세포적증식，병증강기대위암SGC7901세포적살상능력，기궤제가능여상조γδT세포표면적천공소、과립매B、INF-γ、CD107a적표체유관。
Objective To explore the cytotoxicity ofγδT cells against gastric cancer SGC7901 cells after exposed to wogonin and the mechanism involved.Methods Human peripheral γδT cells were proliferated using isopentenyl pyro-phosphate method in vitro.The resultant cells were treated with wogonin at various concentrations and their growth was detected by MTT assay.The amounts of perforin, granzyme B, interferon γ( INF-γ) , and CD107a on the surface ofγδT cells were measured by flow cytometer (FCM) before and after treatment of wogonin.The cytotoxicity ofγδT cells a-gainst SGC7901 cells treated by wogonin was detected by LDH releasing assay.Results The proportion of γδ T cells was increased from 2.51%to 80.12%after exposure to IPP for 10 days.Compared to the control group, 0.1-6.25 mg/L of wogonin could stimulate the growth of γT cells (P<0.05).Meanwhile, wogonin-induced γT cells produced higher levels of f perforin, granzyme B, INF-γ, and CD107a (P<0.05), with enhanced cytotoxicity against SGC7901 cells compared with the control (P<0.05).Conclusion Wogonin can promote the proliferation of T cells at certain concentrations, and enhance their cytotoxicity against SGC7901 cells, which may be related with increased expression of peforin, granzyme B, INF-γ, and CD107a on the surface of these cells.