CAJ | 학술논문

聚-β-羟基丁酸酯（PHB）作为一种完全可降解生物塑料，有望替代传统塑料应用于食品包装、农业和医药等领域，但高昂的生产成本制约着PHB更广泛的应用。本文在以前培养基配方优化结果的基础上，对发酵工艺参数进行响应面优化，确定最佳发酵工艺参数，进一步提高PHB产量。首先，通过单因素试验，确定了转速、初始pH值、接种量和温度的水平范围；然后，以单因素试验结果为基础，采用Design Expert8.0软件设计Box-Behnken四因素三水平、5个中心点、共29组的响应面试验，得到拟合回归方程；最后，通过软件分析确定了假单胞菌koreensis发酵产PHB的最佳工艺参数。结果表明：转速170.91r/min、初始pH值7.64、接种量6.21%和温度32.15℃， PHB产量达4.42g/L，为优化前的1.38倍，效果显著。
취-β-간기정산지（PHB）작위일충완전가강해생물소료，유망체대전통소료응용우식품포장、농업화의약등영역，단고앙적생산성본제약착PHB경엄범적응용。본문재이전배양기배방우화결과적기출상，대발효공예삼수진행향응면우화，학정최가발효공예삼수，진일보제고PHB산량。수선，통과단인소시험，학정료전속、초시pH치、접충량화온도적수평범위；연후，이단인소시험결과위기출，채용Design Expert8.0연건설계Box-Behnken사인소삼수평、5개중심점、공29조적향응면시험，득도의합회귀방정；최후，통과연건분석학정료가단포균koreensis발효산PHB적최가공예삼수。결과표명：전속170.91r/min、초시pH치7.64、접충량6.21%화온도32.15℃， PHB산량체4.42g/L，위우화전적1.38배，효과현저。
As a completely biodegradable plastic, poly-β-hydroxybutyrate ( PHB) is expected to replace traditional plastics used in food packaging, agriculture, medicine, etc., but high cost of production restricts the wider applications of PHB. Based on previous results on media formulations, optimum fermentation parameters were determined by response surface method-ology, and yield of PHB was promoted. First of all, level range of rotation speed, initial pH, inoculation amount and tempera-ture were confirmed; and then, fitted regression equation was gotten based on results of single factor experiments by using Design Expert8.0 to design Box-Behnken response surface experimental; finally, optimum fermentation parameters were determined by software analysis. Results showed that rotation speed 170.91r/min, initial pH 7.64, inoculation amount 6.21%, and temperature 32.15℃, PHB production reached 4.42g/L. Effect was significant that production of PHB was 1.38 times as before.