CAJ | 학술논문

目的：研究右归饮调控辐射后小鼠体内的异体骨髓细胞向成骨细胞分化，尝试揭示其防治辐照引起的骨量丢失的机制。方法：选用80只8 w龄雄性BALB/c小鼠，随机选取32只用于骨髓细胞提取和回输。将剩余48只小鼠随机分成6组，每组8只，分别为空白对照组、辐照组、骨髓细胞（BM）回输组、右归饮高剂量组、中剂量组、低剂量组。辐照组：接受6.0 Gy的γ辐照；BM回输组：6.0 Gy的γ辐照+同种异体骨髓细胞1∶1回输；右归饮高、中、低剂量组：在骨髓回输组的基础上，分别将0.51 g/mL浓度的右归饮每天以0.03 g/mL、0.02 g/mL、0.01 g/mL灌胃。2 w后，检测各组小鼠血清碱性磷酸酶（ALP）、双侧股骨单位体积内骨量（即骨密度）的差异及股骨组织病理变化。结果：右归饮高剂量组双侧股骨骨密度显著高于辐照组、BM回输组（P<0.01），右归饮中剂量组与辐照组、BM回输组比较差异均具有统计学意义（P<0.05）；右归饮高、中剂量组及空白对照组ALP浓度显著高于辐照组（P<0.01），且高剂量组高于低剂量组、BM回输组（P<0.05）。组织病理显示：辐照后骨髓组织结构凌乱，骨髓单核细胞大量消失，部分组织变性，骨小梁稀疏甚至消失，经BM回输后骨小梁重现，骨髓单核细胞数量明显恢复，与BM回输组比较，高剂量组骨小梁排列有序坚实，脂肪空泡数量少，接近空白对照组。结论：右归饮可促进异体骨髓细胞向成骨分化，改善辐照后小鼠骨量丢失情况。
목적：연구우귀음조공복사후소서체내적이체골수세포향성골세포분화，상시게시기방치복조인기적골량주실적궤제。방법：선용80지8 w령웅성BALB/c소서，수궤선취32지용우골수세포제취화회수。장잉여48지소서수궤분성6조，매조8지，분별위공백대조조、복조조、골수세포（BM）회수조、우귀음고제량조、중제량조、저제량조。복조조：접수6.0 Gy적γ복조；BM회수조：6.0 Gy적γ복조+동충이체골수세포1∶1회수；우귀음고、중、저제량조：재골수회수조적기출상，분별장0.51 g/mL농도적우귀음매천이0.03 g/mL、0.02 g/mL、0.01 g/mL관위。2 w후，검측각조소서혈청감성린산매（ALP）、쌍측고골단위체적내골량（즉골밀도）적차이급고골조직병리변화。결과：우귀음고제량조쌍측고골골밀도현저고우복조조、BM회수조（P<0.01），우귀음중제량조여복조조、BM회수조비교차이균구유통계학의의（P<0.05）；우귀음고、중제량조급공백대조조ALP농도현저고우복조조（P<0.01），차고제량조고우저제량조、BM회수조（P<0.05）。조직병리현시：복조후골수조직결구릉란，골수단핵세포대량소실，부분조직변성，골소량희소심지소실，경BM회수후골소량중현，골수단핵세포수량명현회복，여BM회수조비교，고제량조골소량배렬유서견실，지방공포수량소，접근공백대조조。결론：우귀음가촉진이체골수세포향성골분화，개선복조후소서골량주실정황。
Objective:To study the influence of Youguiyin on the osteogenic differentiation of the allogeneic bone marrow stem cell in mice so as to reveal the mechanism of Youguiyin preventing bone loss caused by irradiation. Methods:Eighty BALB/c male mice with age of 8-week were prepared. 32 mice of them were used to extract bone marrow stem cell for transplantation. 48 mice were randomly divided into control group,irradiation group,bone marrow cell(BM)transplanting group,Youguiyin high dose group,middle dose group,low dose group. The mice in irradiation group were exposed to 6.0 Gy gamma irradiation. The mice in BM transplanting group were exposed 6.0 Gy irradiation and received bone marrow cells. The mice in high dose group,middle dose group and low dose group were exposed to 6.0 Gy irradiation and received bone marrow cells and gavaged with Youguiyin of 0.03 mL/g,0.02 mL/g,0.01 mL/g,respectively. After 2 w,serum alkaline phosphatase (ALP),bone volume of femur bone (bone mineral density)and pathological tissue changing were observed. Results:Compared with the irradiation group and BM transplanting group,bone mineral density in high dose group was significantly increased (P<0.01). Middle dose group had a statistically difference with the irradiation group and BM transplanting (P < 0.05).Compared with the irradiation group,in high does group,middle dose group and control group ALP had increased (P<0.01)and high does group had a statistically difference with low dose group and BM transplanting group (P<0.05). Femur pathology:After irradiating,the structure of myeloid tissue become mass and monocyte was rare in the bone marrow and a part of tissue were degenerated. Bone trabeculas reappeared and bone marrow mononuclear cells were recovered,when injected with bone marrow stem cell. In addition,high dose group has strong and well ordered trabecular,less fat cavitations. Conclusion:Youguiyin can promote the osteogenic differentiation of the allogeneic bone marrow stem cell to cure the bone loss caused by irradiation.