CAJ | 학술논문

建立HPLC法测定骨康胶囊中含芍药苷量的方法．样品经甲醇超声提取后，采用HPLC法测定骨康胶囊中含芍药苷量的方法，使用C18色谱柱，甲醇－水（28∶72）为流动相．检测波长为230 nm，流速为1．0 mL／min．芍药苷线性范围是9．8～99．0μg／mL，相关系数r＝0．9998，平均回收率99．46％．样品处理方法合理，方法学考察符合定量要求，结果准确，可用于骨康胶囊中含芍药苷的量测定．
건립HPLC법측정골강효낭중함작약감량적방법．양품경갑순초성제취후，채용HPLC법측정골강효낭중함작약감량적방법，사용C18색보주，갑순－수（28∶72）위류동상．검측파장위230 nm，류속위1．0 mL／min．작약감선성범위시9．8～99．0μg／mL，상관계수r＝0．9998，평균회수솔99．46％．양품처리방법합리，방법학고찰부합정량요구，결과준학，가용우골강효낭중함작약감적량측정．
To establish an HPLC method for the determination of paeoniflorin in Gukang cap-sules.The sample was extracted in methanol by ultrasonic .The paeoniflorin content of Gu-kang capsules was determined on C 18 chromatographic column using HPLC .Methanol -wa-ter (28∶72) was used as the mobile phase using .The detection wavelength was 230 nm. The flow rate was 1.0 mL/min.Paeoniflorin linear range was 9.8 ~99.0 μg/mL, and the correlation coefficient r was 0.999 8.And the average recovery rate was 99.46%.The pro-cessing method is reasonable .The methodology conforms to the requirements of the quantita-tive.This method can be used for the determination of paeoniflor in Gukang capsules .