CAJ | 학술논문

目的：观察赖氨大黄酸（ RHL）对百草枯中毒小鼠心脏损伤的保护作用及其机制。方法小鼠随机分为对照组、百草枯模型组（腹腔注射百草枯建立活性氧自由基引起心脏损伤的小鼠模型）及RHL治疗组（造模前1周给予RHL（50 mg／kg）。用硫代巴比妥酸法检测心脏组织MDA含量，用联苯三酚自氧化法检测SOD活性，用NADPH偶联法检测GSH-Px活性。 HE染色及DCFH-DA染色分别观察心脏病理改变及活性氧水平；Western blot 检测心肌衰老相关基因的表达。结果与对照组相比，百草枯模型组小鼠心脏组织SOD和GSH-Px活性下降（ P＜0.05），MDA水平升高（ P＜0.05），RHL能减轻百草枯的作用（ P＜0.05）；百草枯引起心肌结构破坏，心肌组织活性氧水平升高， RHL减轻上述变化（ P＜0.05）。百草枯能够降低SIRT1的表达，增加P53的乙酰化及P53和P66的表达。 RHL减轻上述变化（ P＜0.05）。结论 RHL可以减轻百草枯中毒后所致的小鼠心脏损伤。
목적：관찰뢰안대황산（ RHL）대백초고중독소서심장손상적보호작용급기궤제。방법소서수궤분위대조조、백초고모형조（복강주사백초고건립활성양자유기인기심장손상적소서모형）급RHL치료조（조모전1주급여RHL（50 mg／kg）。용류대파비타산법검측심장조직MDA함량，용련분삼분자양화법검측SOD활성，용NADPH우련법검측GSH-Px활성。 HE염색급DCFH-DA염색분별관찰심장병리개변급활성양수평；Western blot 검측심기쇠로상관기인적표체。결과여대조조상비，백초고모형조소서심장조직SOD화GSH-Px활성하강（ P＜0.05），MDA수평승고（ P＜0.05），RHL능감경백초고적작용（ P＜0.05）；백초고인기심기결구파배，심기조직활성양수평승고， RHL감경상술변화（ P＜0.05）。백초고능구강저SIRT1적표체，증가P53적을선화급P53화P66적표체。 RHL감경상술변화（ P＜0.05）。결론 RHL가이감경백초고중독후소치적소서심장손상。
Objective To investigate the protective effects of rhein lysinate ( RHL) on cardiac tissue damage in-duced by paraquat in experimental mice , and to clarify its mechanism .Methods In this study mice were assigned to the following three groups: control, paraquat model, and RHL-treated groups.The model of oxidative damage mice was established by intraperitoneal injection of paraquat .RHL-treated group was given RHL ( 50 mg/kg ) by gavage for one week before performing model .The other two groups were given equal volume of distilled water .For making model , paraquat was intraperitoneally injected in the paraquat model and RHL-treated group .The content of MDA was detected by thiobarbituric acid assay .The activities of SOD and GSH-Px were detected by biphenyl three phenolic autoxidation assay and NADPH coupling method respectivly .The pathological profile of cardiac tis-sue was observed by hematoxylin and eosin ( HE) staining and reactive oxygen species was observed by DCFH-DA staining .The change of proteins related to myocardial damage detected by Western blot .Results Compared with control group, the activities of SOD and GSH-Px decreased (P<0.05) and the content of MDA increased (P<0.05) in paraquat model group .However , these changes were attenuated byr RHL treatmen ( P<0.05 ) .The pathologi-cal examination indicated the structure of cardiac tissue was damaged and reactive oxygen species of cardiac tissue was increased after paraquat was given , however , these changes were attenuated after RHL treatmen .It was shown in western blot analysis that compared with control group , the expression of SIRT1 decreased, the acetylation of P53 and the expression of P 53 and P66 increased in paraquat-treated group .These changes were attenuated by RHL treatmen ( P<0.05 ) .Conclusions RHL may attenuate paraquat-induced cardiac injury in mice .