山西中医学院学报
山西中醫學院學報
산서중의학원학보
Journal of Shanxi College of Traditional Chinese Medicine
2015年
4期
13-15
,共3页
肺癌%脾虚%加味黄芪建中汤%HIF-1α%V-ATPase c%微环境
肺癌%脾虛%加味黃芪建中湯%HIF-1α%V-ATPase c%微環境
폐암%비허%가미황기건중탕%HIF-1α%V-ATPase c%미배경
lung carcinoma%spleen deficiency syndrome%modified Huangqi Jianzhong decoction%HIF-1α%V-ATPase c%microenvironment
目的：探讨加味黄芪建中汤对脾虚证肺癌小鼠肿瘤转移及HIF-1α、V-ATPase c mRNA表达的影响。方法：复因素法建立脾虚证小鼠模型，进行分组并接种Lewis肺癌瘤株后的第10天，取肺及肿瘤组织，压片法计肺转移结节数，RT-PCR法检测V-ATPase c mRNA的表达，Power Vision法检测HIF-1α的表达。结果：与肺癌对照组比较，肺癌脾虚组小鼠肺转移结节数增多（P＜0.05），与脾虚肺癌组比较，脾虚肺癌治疗组肺转移结节数减少，HIF-1α表达下调（P＜0.01）。结论：加味黄芪建中汤对小鼠肿瘤转移有抑制作用，其机制可能与肿瘤微环境的HIF-1α表达下调有关。
目的：探討加味黃芪建中湯對脾虛證肺癌小鼠腫瘤轉移及HIF-1α、V-ATPase c mRNA錶達的影響。方法：複因素法建立脾虛證小鼠模型，進行分組併接種Lewis肺癌瘤株後的第10天，取肺及腫瘤組織，壓片法計肺轉移結節數，RT-PCR法檢測V-ATPase c mRNA的錶達，Power Vision法檢測HIF-1α的錶達。結果：與肺癌對照組比較，肺癌脾虛組小鼠肺轉移結節數增多（P＜0.05），與脾虛肺癌組比較，脾虛肺癌治療組肺轉移結節數減少，HIF-1α錶達下調（P＜0.01）。結論：加味黃芪建中湯對小鼠腫瘤轉移有抑製作用，其機製可能與腫瘤微環境的HIF-1α錶達下調有關。
목적：탐토가미황기건중탕대비허증폐암소서종류전이급HIF-1α、V-ATPase c mRNA표체적영향。방법：복인소법건립비허증소서모형，진행분조병접충Lewis폐암류주후적제10천，취폐급종류조직，압편법계폐전이결절수，RT-PCR법검측V-ATPase c mRNA적표체，Power Vision법검측HIF-1α적표체。결과：여폐암대조조비교，폐암비허조소서폐전이결절수증다（P＜0.05），여비허폐암조비교，비허폐암치료조폐전이결절수감소，HIF-1α표체하조（P＜0.01）。결론：가미황기건중탕대소서종류전이유억제작용，기궤제가능여종류미배경적HIF-1α표체하조유관。
Objective:To explore effects of modified Huangqi Jianzhong decoction on tumor metastasis,expressions of HIF-1α,V-ATPase c mRNA in lung carcinoma model mice with spleen deficiency syndrome. Method:The model mice with spleen deficiency syndrome was established by multifactor method. On the 10th day,pulmonary metastatic nodules were counted,expressions of V-ATPase c mRNA and HIF-1α were detected. Result:Compared with lung carcinoma with spleen deficiency group,pulmonary metastasis nodules and expression of HIF-1α were decreased in lung carcinoma with spleen deficiency treatment group (P<0.05). Conclusion:Modified Huangqi Jianzhong decoction can inhibit tumor metastasis,which may be associated with down-regulating of HIF-1αin tumor microenvironment.
