CAJ | 학술논문

目的：探讨尿路上皮癌相关1（ UCA1）在胰腺癌中的表达及其对胰腺癌细胞侵袭转移的影响。方法用荧光定量PCR检测11例胰腺癌组织和配对的癌旁组织及5种胰腺癌细胞系中UCA1的表达，通过RNA干扰技术降低胰腺癌细胞系BxPC-3的UCA1水平，用Transwell侵袭实验和划痕实验观察BxPC-3细胞的侵袭和迁移能力，West-ern blot检测MMP-2和MMP-9的蛋白水平。结果胰腺癌组织的UCA1水平高于相应的癌旁组织（ P＜0.05）， UCA1差异性表达于5种胰腺癌细胞系；干扰UCA1后， BxPC-3细胞的MMP-2和MMP-9的蛋白水平均降低（ P＜0.01），侵袭能力和迁移能力明显减弱（ P＜0.01）。结论 UCA1在胰腺癌中高表达，下调UCA1通过降低MMP-2和MMP-9的表达减弱胰腺癌细胞系BxPC-3的体外侵袭转移能力。
목적：탐토뇨로상피암상관1（ UCA1）재이선암중적표체급기대이선암세포침습전이적영향。방법용형광정량PCR검측11례이선암조직화배대적암방조직급5충이선암세포계중UCA1적표체，통과RNA간우기술강저이선암세포계BxPC-3적UCA1수평，용Transwell침습실험화화흔실험관찰BxPC-3세포적침습화천이능력，West-ern blot검측MMP-2화MMP-9적단백수평。결과이선암조직적UCA1수평고우상응적암방조직（ P＜0.05）， UCA1차이성표체우5충이선암세포계；간우UCA1후， BxPC-3세포적MMP-2화MMP-9적단백수평균강저（ P＜0.01），침습능력화천이능력명현감약（ P＜0.01）。결론 UCA1재이선암중고표체，하조UCA1통과강저MMP-2화MMP-9적표체감약이선암세포계BxPC-3적체외침습전이능력。
Objective To explore the expression of urothelial carcinoma-associated 1 ( UCA1 ) in pancreatic cancer cell lines and its influence on the invasion and metastasis of the pancreatic cancer cells .Methods The expression of UCA1 in pancreatic cancer tissues and paired adjacent normal tissues ( 11 cases ) and 5 pancreatic cancer cell lines was analyzed by real-time PCR.The level of UCA1 in BxPC-3 was knocked down by small interfering RNA . The ability of invasion and migration in vitro of transfected BxPC-3 was detected by Transwell invasion assay and wound healing assay .The protein levels of MMP-2 and MMP-9 were measured by Western blot experiment .Results The expression level of UCA1 in pancreatic cancer tissues was higher than that in paired adjacent normal tissues , and UCA1 differentially expressed in 5 pancreatic cancer cell lines .Down-regulation of UCA1 by siRNA suppressed the expression of MMP-2 and MMP-9 in BxPC3, and dramatically impaired the ability of invasion and migration of BxPC-3.Conclusions UCA1 is over-expressed in pancreatic cancer , and down-regulation of UCA1 attenuates the capacity of invasion and metastasis in vitro of BxPC-3 by decreasing MMP-2 and MMP-9.