CAJ | 학술논문

建立高效液相法同时测定昙花中含槲皮素、山奈酚和异鼠李素的量．运用Diamonsil C18色谱柱为分析柱，以甲醇－0．4％磷酸水溶液（55∶45）为流动相，流速为1．0 mL／min，检测波长为360 nm．测得槲皮素在5．5～88μg／mL范围内呈良好线性关系，r＝0．9999（n＝6）；山奈酚在4～64μg／mL范围内呈良好线性关系，r＝0．9999（n＝6）；异鼠李素在6．5～104μg／mL范围内呈良好线性关系，r＝0．9999（n＝6），三者的回收率分别为（99．84±0．23）％（RSD＝0．24％）、（100．20±0．52）％（RSD＝0．38％）、（99．99±0．12）％（RSD＝0．11％）．槲皮素、异鼠李素和山奈酚的量分别为0．05、0．026、0．039 mg／g．本方法可同时测定昙花中槲皮素、异鼠李素和山奈酚，操作简单、灵敏、重复性好，适用于昙花及其制剂的质量控制．
건립고효액상법동시측정담화중함곡피소、산내분화이서리소적량．운용Diamonsil C18색보주위분석주，이갑순－0．4％린산수용액（55∶45）위류동상，류속위1．0 mL／min，검측파장위360 nm．측득곡피소재5．5～88μg／mL범위내정량호선성관계，r＝0．9999（n＝6）；산내분재4～64μg／mL범위내정량호선성관계，r＝0．9999（n＝6）；이서리소재6．5～104μg／mL범위내정량호선성관계，r＝0．9999（n＝6），삼자적회수솔분별위（99．84±0．23）％（RSD＝0．24％）、（100．20±0．52）％（RSD＝0．38％）、（99．99±0．12）％（RSD＝0．11％）．곡피소、이서리소화산내분적량분별위0．05、0．026、0．039 mg／g．본방법가동시측정담화중곡피소、이서리소화산내분，조작간단、령민、중복성호，괄용우담화급기제제적질량공제．
A rapid and sensitive HPLC method was developed to detect the content of querce-tin, kaempferol and isorhamnetin in Epiphyllum oxypetalum .Diamond -C18 column was used as chromatographic column , methanol-0.4%phosphoric acid (55∶45) water solution as mobile phase , and the detection wavelength was set at 360 nm, with a flow rate at 1.0 mL/min.There was a good linear relationship when quercetin was in the range of 5.5~88μg/mL, r=0.999 9(n=6), kaempferol was in the range of 4~64 μg/mL, r=0.999 9(n=6) and isorhamnetin was in the range of 6.5~104 μg/mL, r=0.999 9(n=6).The av-erage recoveries were (99.84 ±0.23)% ( RSD=0.24%), (100.20 ±0.52)% ( RSD=0.38%), 99.99 ±0.12% (RSD=0.11%) respectively.The result showed that the con-tent of quercetin, kaempferol and isorhamnetin in Epiphyllum oxypetalum were 0.05, 0. 026, 0.039 mg/g, respectively.The established method was simple , sensitive and repro-ducible to detect the content of quercetin , kaempferol and isorhamnetin in Epiphyllum oxyp-etalum at the same time , which could be applied in the quality control of Epiphyllum oxyp-etalum and its preparations .