CAJ | 학술논문

目的：观察表没食子儿茶素没食子酸酯（ EGCG）对细菌脂多糖（ LPS）所致大鼠神经胶质细胞炎症的保护作用。方法取新生乳鼠原代神经胶质细胞培养，用LPS激活小胶质细胞引起炎性反应。高效液相色谱检测细胞兴奋性／抑制氨基酸含量，用酶联免疫吸附实验（ELISA）和蛋白免疫印记试验（Western blot）检测炎性因子蛋白含量。结果 LPS激活神经小胶质细胞，大幅上调TNF-α、IL-1β及IL-8炎性因子和升高iNOS蛋白质水平（ P＜0.05）， EGCG明显抑制这些炎性因子的过度产生（ P＜0.05），同时还显著降低Glu和升高γ-GABA的浓度（ P＜0.05）。结论 EGCG能减弱LPS引起的体外培养神经胶质细胞的炎性反应。
목적：관찰표몰식자인다소몰식자산지（ EGCG）대세균지다당（ LPS）소치대서신경효질세포염증적보호작용。방법취신생유서원대신경효질세포배양，용LPS격활소효질세포인기염성반응。고효액상색보검측세포흥강성／억제안기산함량，용매련면역흡부실험（ELISA）화단백면역인기시험（Western blot）검측염성인자단백함량。결과 LPS격활신경소효질세포，대폭상조TNF-α、IL-1β급IL-8염성인자화승고iNOS단백질수평（ P＜0.05）， EGCG명현억제저사염성인자적과도산생（ P＜0.05），동시환현저강저Glu화승고γ-GABA적농도（ P＜0.05）。결론 EGCG능감약LPS인기적체외배양신경효질세포적염성반응。
Objective To determine the effects of EGCG on lipopolysaccharide ( LPS)-induced neuroinflamma-tion and investigate the role of neuroprotection mediated by EGCG .Methods Primary cultures of rat gliacyte were used as an in vitro model to examine the effects of EGCG on LPS-induced neuronal damage .The intracellu-lar Glu andγ-GABA were quantified via HPLC .Then the protein level of TNF-α,IL-1βand IL-8 was determined by ELISA and Western blot assay .Results Compared with the control group , LPS apparently induced the pro-duction of intracellular ROS ( P<0.05 ) and released the TNF-α, IL-1βand IL-8 in the primary cultures super-natant (P<0.05).Compared with the LPS group,EGCG significantly attenuated the release of TNF-α, IL-1βand IL-8 ( P<0.05 ) and the level of iNOS protein ( P<0.05 ) .LPS apparently induced the production of intra-cellular ROS( P<0.05 ) and released the TNF-α, IL-1βand IL-8 in the primary cultures supernatant ( P <0.05 ) .EGCG significantly attenuated the release of TNF-α, IL-1βand IL-8 ( P<0.05 ) and the level of iNOS protein(P<0.05), and rugulated the concentration of Glu/γ-GABA(P<0.05).Conclusions EGCG is effective in protecting hosts against LPS-induced neuroinflammation through anti-inflammatory effects and regulating extracel-lular Amino acid levels .