CAJ | 학술논문

以含5 mg·L－1潮霉素 MS 培养基进行抗性筛选培养，以 EHA105农杆菌介异，将龙眼2个非光合铁氧还蛋白基因导入文心兰，结果表明，所获 Fd3转化植株表现更高的移栽成活率和对抗病害的能力。与野生型植株相比，Fd3转化植株抵抗软腐病能力提高，由高感软腐病（Di ＝100）提升为中抗软腐病（Di ＝27．5），移栽成活率由79．3％提高至95．7％，均达极显著差异水平。
이함5 mg·L－1조매소 MS 배양기진행항성사선배양，이 EHA105농간균개이，장용안2개비광합철양환단백기인도입문심란，결과표명，소획 Fd3전화식주표현경고적이재성활솔화대항병해적능력。여야생형식주상비，Fd3전화식주저항연부병능력제고，유고감연부병（Di ＝100）제승위중항연부병（Di ＝27．5），이재성활솔유79．3％제고지95．7％，균체겁현저차이수평。
To study the enhancing effect on the disease-resistance of longan by genetic modification,twonon-photosynthetic ferredoxins from the plant were transferred to Oncdium through Agrobacterium tumefaciens strain EHA105 for cultivation on a culture medium containing 5 mg·L-1 hygromycin.As compared to the plants in the wild,the Fd3 transgenic plants showed a significantly higher resistance to soft rot disease improving the resistance grading from highly sensitive (Di=100)to moderate (Di=27.5).In addition,the survival rate of the transplants also increased significantly from 79.3% to 95.7%.