CAJ | 학술논문

目的：体外探讨Notch信号通路对膀胱癌细胞侵袭性与耐药性的影响及分子机制。方法采用Notch信号通路受体完全阻断剂（γ分泌酶抑制剂）处理膀胱癌T24、5637和J82细胞48 h后，倒置显微镜观察膀胱癌细胞增生及形态；用RT-PCR和Western blot在mRNA和蛋白水平检测上皮-间质转化（ EMT）分子标志物E-cadherin、N-cad-herin、vimentin和Alpha-smooth muscle actin的表达；MTT、Transwell检测膀胱癌细胞耐药性及侵袭能力。结果完全阻断Notch信号通路后，镜下显示膀胱癌细胞形态变小，细胞分散；EMT分子标志物E-cadherin mRNA和蛋白水平表达上调（ P＜0.05），N-cadherin、vimentin、Alpha-smooth muscle actin mRNA 和蛋白水平表达下调（ P＜0.05）；膀胱癌细胞T24、5637和J82增殖明显被抑制（ P＜0.05）；膀胱癌细胞T24、5637和J82穿过微孔膜的细胞数明显减少（ P＜0.05）。结论 Notch信号通路可通过调控EMT的变化而改变膀胱癌的侵袭性与耐药性。
목적：체외탐토Notch신호통로대방광암세포침습성여내약성적영향급분자궤제。방법채용Notch신호통로수체완전조단제（γ분비매억제제）처리방광암T24、5637화J82세포48 h후，도치현미경관찰방광암세포증생급형태；용RT-PCR화Western blot재mRNA화단백수평검측상피-간질전화（ EMT）분자표지물E-cadherin、N-cad-herin、vimentin화Alpha-smooth muscle actin적표체；MTT、Transwell검측방광암세포내약성급침습능력。결과완전조단Notch신호통로후，경하현시방광암세포형태변소，세포분산；EMT분자표지물E-cadherin mRNA화단백수평표체상조（ P＜0.05），N-cadherin、vimentin、Alpha-smooth muscle actin mRNA 화단백수평표체하조（ P＜0.05）；방광암세포T24、5637화J82증식명현피억제（ P＜0.05）；방광암세포T24、5637화J82천과미공막적세포수명현감소（ P＜0.05）。결론 Notch신호통로가통과조공EMT적변화이개변방광암적침습성여내약성。
Objective To investigate the effect of notch signaling pathway on drug resistance and invasiveness of bladder cancer .Methods We observed the changes of growth and morphology of bladder cancer T 24 , 5637 and J82 cells which treated for 48 hours using γ-secretase inhibitor by inverted microscope .The mRNA and protein lev-els of the EMT molecular markers , including E-cadherin , N-cadherin , vimentin and Alpha-smooth muscle actin were examined by RT-PCR and Western blot in bladder cancer cells;Detected the changes of drug resistance and invasion respectively by MTT and Transwell in bladder cancer cells .Results After completely blocking the Notch signaling pathway , the inverted microscope showed that bladder cancer cells became smaller and more disperse ;RT-PCR and Western blot showed the mRNA and protein levels of E-cadherin were up-regulated ( P<0.05 ) , contrast , N-cadherin , vimentin and Alpha-smooth muscle actin were down-regulated ( P<0.05 ); The prolifera-tion of bladder cancer cells were significantly inhibited by MTT test;The number of through microporous membrane cells significantly decreased ( P<0.05 ) shown by Transwell test .Conclusions The Notch signaling pathway is completely blocked that nhibites proliferation and EMT of bladder cancer cells , reduces drug resistance and inva-sion in bladder cancer cells .It suggests that drug resistance and invasiveness of bladder cancer can be changed through EMT which is regulated through notch signaling pathway .