CAJ | 학술논문

目的：观察斑蝥素酸镁、斑蝥素及斑蝥素酸钠3种斑蝥素类物质对人肝癌细胞HepG2的体外抗肿瘤活性。方法（1）采用磺酰罗丹明染色法（SRB法）检测不同浓度斑蝥素酸镁、斑蝥素及斑蝥素酸钠在体外对人肝癌细胞HepG2增殖的抑制作用；（2）使用透射电镜观察2.27μmol/L斑蝥素酸镁作用于肝癌细胞HepG248 h后细胞超显微结构的变化。结果（1）斑蝥素酸镁、斑蝥素及斑蝥素酸钠对人肝癌细胞HepG2有明显抑制作用，抑制率随药物浓度的增加而升高，且呈剂量效应关系，其半数抑制浓度（IC50）分别为2.27、5.70、8.41μmol/L；（2）透射电镜下见核染色质聚集成块附着于核膜下，且见核异形等凋亡细胞形态特征。结论斑蝥素酸镁对人肝癌细胞HepG2的抑制作用优于斑蝥素和斑蝥素酸钠，且斑蝥素酸镁可以诱导人肝癌细胞HepG2发生凋亡。
목적：관찰반모소산미、반모소급반모소산납3충반모소류물질대인간암세포HepG2적체외항종류활성。방법（1）채용광선라단명염색법（SRB법）검측불동농도반모소산미、반모소급반모소산납재체외대인간암세포HepG2증식적억제작용；（2）사용투사전경관찰2.27μmol/L반모소산미작용우간암세포HepG248 h후세포초현미결구적변화。결과（1）반모소산미、반모소급반모소산납대인간암세포HepG2유명현억제작용，억제솔수약물농도적증가이승고，차정제량효응관계，기반수억제농도（IC50）분별위2.27、5.70、8.41μmol/L；（2）투사전경하견핵염색질취집성괴부착우핵막하，차견핵이형등조망세포형태특정。결론반모소산미대인간암세포HepG2적억제작용우우반모소화반모소산납，차반모소산미가이유도인간암세포HepG2발생조망。
Objective To investigate the in vitro anti-tumor activity of three kinds of cantharidin materials magnesium cantharidate,cantharidin and sodium cantharidate on hepatocellular carcinoma cells HepG2. Methods (1)The sulforhodamine B (SRB) assay was employed to evaluate the inhibitory effect of magnesium cantharidate ,cantharidin and sodium cantharidate on hepatocellular carcinoma cells HepG2 in vitro;(2)the transmission electron microscopy was used to observe the ultra-structural changes after 2.27 μmol/L magnesium cantharidate acting on hepatocellular carcinoma cells HepG2 for 48 h. Results (1) Magnesium cantharidate,cantharidin and sodium cantharidate had obvious inhibitory effects on the proliferation of hepatocellular carcinoma cells HepG2,the inhibitory rate was increased with the medication concentration increase ,showing the dose-effect relationship,the half inhibitory concentrations(IC50) were 2.27,5.70,8.41 μmol/L,respectively;(2)the transmission electron microscopy found the morphological features of apoptotic cells ,such as the nuclear chromatin was gathered into a block attaching to below the nuclear membrane,heteromorphic nucleus and so on. Conclusion The inhibiting effect of magnesium cantharidate on hepatocellular carcinoma cells HepG2 is superior to that of cantharidin and sodium cantharidate,moreover magnesium cantharidate can induce the apoptosis of hepatocellular carcinoma cells HepG 2 .