CAJ | 학술논문

目的：探讨CCK-8对LPS激活RAW264.7细胞CHOP／caspase-11／caspase-1信号通路的影响。方法用LPS和（或） CCK-8和（或） CCK-8的1受体阻滞剂孵育RAW 264.7细胞，用Western blot法检测CHOP、caspase-11蛋白表达，用试剂盒检测caspase-1活性，用ELISA技术检测细胞培养上清中IL-1β水平。结果与对照组相比，LPS组CHOP、caspase-11表达水平均显著升高（ P＜0.05），给予CCK-8可有效抑制LPS诱导的CHOP、caspase-11表达升高（ P＜0.05），而预先给予CCK-8的1受体阻滞剂可明显抑制CCK-8的作用。 caspase-1活性变化、IL-1β含量变化趋势均与CHOP、caspase-11表达水平变化趋势一致（ P＜0.05）。结论 CCK-8通过受体1抑制CHOP／caspase-11／caspase-1表达，减少LPS诱导IL-1β的生成，发挥抗炎作用。
목적：탐토CCK-8대LPS격활RAW264.7세포CHOP／caspase-11／caspase-1신호통로적영향。방법용LPS화（혹） CCK-8화（혹） CCK-8적1수체조체제부육RAW 264.7세포，용Western blot법검측CHOP、caspase-11단백표체，용시제합검측caspase-1활성，용ELISA기술검측세포배양상청중IL-1β수평。결과여대조조상비，LPS조CHOP、caspase-11표체수평균현저승고（ P＜0.05），급여CCK-8가유효억제LPS유도적CHOP、caspase-11표체승고（ P＜0.05），이예선급여CCK-8적1수체조체제가명현억제CCK-8적작용。 caspase-1활성변화、IL-1β함량변화추세균여CHOP、caspase-11표체수평변화추세일치（ P＜0.05）。결론 CCK-8통과수체1억제CHOP／caspase-11／caspase-1표체，감소LPS유도IL-1β적생성，발휘항염작용。
Objective To investigate the effect of CCK-8 on CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264.7 cells.Methods RAW264.7 cells were incubated with LPS and/or CCK-8 and/or CCK-8 1R blocking agent devazepide for indicated times .CHOP and caspase-11 protein expressions were determined by West-ern blot , caspase-1 activity was analyzed by kit , IL-1βexpression level was analyzed by ELISA .Results Both CHOP and caspase-11 expression were upregulated in LPS-activated RAW264.7 cell, which were inhibited by pre-treatment of CCK-8 .Pre-treatment of devazepide inhibited the effects of CCK-8 significantly .The same effects were found in caspase-1 activity and IL-1βexpression .Conclusions CCK-8 exerts an anti-inflammatory effect by inhib-iting CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264.7 cell.CCK 1R may contribute to the anti-inflammatory effect of CCK-8.