CAJ | 학술논문

目的：探索载有槲皮素的PEG-PE聚合物胶束（ M-Q）能否在体外逆转耐阿霉素人乳腺癌细胞（ MCF-7 ADRr）的耐药性。方法将PEG-PE聚合物与槲皮素混合制备脂膜，加0.9％氯化钠注射液形成胶束，用动态光散射粒径仪表征其尺寸分布，并将其和阿霉素与MCF-7 ADRr细胞共孵育72 h，用MTS方法检测对肿瘤细胞的杀伤率。结果PEG-PE聚合物胶束能有效运载槲皮素，其包封率高达74％以上，平均粒径为11.11 nm左右，与游离槲皮素相比， M-Q能在体外更大程度地逆转MCF-7 ADRr细胞对于阿霉素的耐药性（ P＜0.05），且PEG-PE胶束自身对靶细胞无明显杀伤作用。结论载有槲皮素的PEG-PE胶束在逆转肿瘤耐药性方面具有应用潜能。
목적：탐색재유곡피소적PEG-PE취합물효속（ M-Q）능부재체외역전내아매소인유선암세포（ MCF-7 ADRr）적내약성。방법장PEG-PE취합물여곡피소혼합제비지막，가0.9％록화납주사액형성효속，용동태광산사립경의표정기척촌분포，병장기화아매소여MCF-7 ADRr세포공부육72 h，용MTS방법검측대종류세포적살상솔。결과PEG-PE취합물효속능유효운재곡피소，기포봉솔고체74％이상，평균립경위11.11 nm좌우，여유리곡피소상비， M-Q능재체외경대정도지역전MCF-7 ADRr세포대우아매소적내약성（ P＜0.05），차PEG-PE효속자신대파세포무명현살상작용。결론재유곡피소적PEG-PE효속재역전종류내약성방면구유응용잠능。
Objective To explore whether quercetin-loaded PEG-PE micelles(M-Q) can synergize the growth-in-hibitory activity of adriamycin prepared ( ADR) by reversing the drug resistance of MCF-7 ADRr breast cancer cells in vitro.Methods M-Q was prepared by adding saline to lipid film containing quercetin and PEG-PE.The size of M-Q was characterized by dynamic light scattering ( DLS) .The inhibition of MCF-7 ADRr cells was evaluated by MTS assay after incubation with M-Q and ADR.Results The incorporation efficiency of quercetin by the micelles was above 74%.The average size of M-Q was 11.11 nm.Compared with the quercetin dissolved in ethanol , M-Q more effectively reversed the drug resistance of MCF-7 ADRr cells in vitro.Conclusions PEG-PE micelles may potentially deliver quercetin to cancer cells for reversal of drug resistance .