CAJ | 학술논문

中药天麻背景复杂，对天麻素的激发-发射光谱(270nm/295nm)干扰特别大。本文利用三维荧光结合化学计量学二阶校正自加权交替三线性( SWATLD)算法对其进行解析，在不经分离的情况下测定了中药天麻中天麻素的含量。计算得到中药天麻中天麻素的平均含量是0．3766%，平均回收率为104．6%，分辨得到的天麻素解析光谱与真实光谱几乎完全重合。说明这种方法可用于中药天麻中天麻素含量的测定。
중약천마배경복잡，대천마소적격발-발사광보(270nm/295nm)간우특별대。본문이용삼유형광결합화학계량학이계교정자가권교체삼선성( SWATLD)산법대기진행해석，재불경분리적정황하측정료중약천마중천마소적함량。계산득도중약천마중천마소적평균함량시0．3766%，평균회수솔위104．6%，분변득도적천마소해석광보여진실광보궤호완전중합。설명저충방법가용우중약천마중천마소함량적측정。
There is a complex background in gastrodia elata Bl. . It has an extremely large interference to the excitation and emission spectra(270 nm/295 nm) of gastrodin. A simple method was developed for direct determination of gastrodin in gastrodia elata Bl. even in the presence of other uncalibrated interferents by using excitation–emission matrix( EEM) fluorescence coupled with second-order calibration based on self-weighted alternating trilinear decomposition( SWATLD) algorithm. The determination results show that the content of gastrodin is 0. 3766%, the average recovery is 104. 6%. The resolved gastrodin spectra and real spectra matched quite well with each other. The experimental results show that the proposed method could be applied to determine quantita-tively gastrodin in gastrodia elata Bl.