CAJ | 학술논문

以麻疯树果壳为原料,采用超声波辅助法提取麻疯树果壳中的多糖。在单因素实验的基础上,利用正交实验优化提取条件,并采用还原能力、DPPH·有机自由基的清除能力、羟自由基的清除能力作为麻疯树果壳多糖的体外抗氧化作用评价的3个指标。结果表明,超声波辅助提取麻疯树果壳多糖的最佳工艺条件为超声功率160 W,提取温度80℃,提取时间1．0 h,料液比1∶25(g·mL-1),在此条件下多糖得率为5．96%。抗氧化实验表明,麻疯树果壳多糖具有良好的还原能力,且清除·OH及DPPH·的能力与浓度呈正相关。当麻疯树果壳多糖浓度为0．5 mg·mL-1时,对·OH 及 DPPH·的清除率分别达到70%和60%。因此,该本方法操作简便、高效,测定快速。
이마풍수과각위원료,채용초성파보조법제취마풍수과각중적다당。재단인소실험적기출상,이용정교실험우화제취조건,병채용환원능력、DPPH·유궤자유기적청제능력、간자유기적청제능력작위마풍수과각다당적체외항양화작용평개적3개지표。결과표명,초성파보조제취마풍수과각다당적최가공예조건위초성공솔160 W,제취온도80℃,제취시간1．0 h,료액비1∶25(g·mL-1),재차조건하다당득솔위5．96%。항양화실험표명,마풍수과각다당구유량호적환원능력,차청제·OH급DPPH·적능력여농도정정상관。당마풍수과각다당농도위0．5 mg·mL-1시,대·OH 급 DPPH·적청제솔분별체도70%화60%。인차,해본방법조작간편、고효,측정쾌속。
Using Jatropha curcas L. shell as material,ultrasonic-assisted technology was applied in the experiment to extract polysac-charides from Jatropha curcas L. shell. The optimal extraction conditions were obtained through single factor test and orthogonal test. Besides,antioxidant activities of the polysaccharides in vitro were investigated by scavenging DPPH·,reducing activity and hy-droxyl free radicals abilities. The results demonstrated that the ultrasonic-assisted extraction optimum technological conditions were as follows:ultrasonic power 160 W,extraction time 1. 0 h,extraction temperature 80℃,ratio of material to solvent 1∶25(g·mL-1) . Under these conditions,the yield of polysaccharides was 5. 96%. The antioxidation experiments indicated that the polysaccharides showed good reducing power activity,also both the scavenging activity of·OH and DPPH·showed concentration dependant. At the concentration of 0. 5 mg·mL-1 ,the·OH and DPPH scavenging rate were 70%and 60%. So,the method was simple,efficient and quick determination.