CAJ | 학술논문

目的：探索miR-34a-5p对K562细胞红系分化的影响。方法分别用miR-34a-5p模拟物和反义抑制寡核苷酸转染K562细胞，用real-time PCR法检测过表达或干扰效率，并进一步用流式细胞术和联苯胺染色法检测K562细胞向红系的分化情况；通过Western blot方法检测miR-34a-5p的靶基因。结果 miR-34a-5p在K562细胞红系分化过程中呈现表达下降趋势；在K562细胞中过表达 miR-34a-5p 可抑制 hemin 诱导的红系分化（ P ＜0.05）；反之，干扰K562内源的miR-34a-5p表达会对K562红系分化产生促进作用（ P＜0.01）；另一方面，miR-34a-5p通过靶向抑制c-MYB的表达抑制细胞向红系分化。结论 miR-34a-5p通过抑制c-MYB在K562细胞早期红系分化过程中发挥促进作用。
목적：탐색miR-34a-5p대K562세포홍계분화적영향。방법분별용miR-34a-5p모의물화반의억제과핵감산전염K562세포，용real-time PCR법검측과표체혹간우효솔，병진일보용류식세포술화련분알염색법검측K562세포향홍계적분화정황；통과Western blot방법검측miR-34a-5p적파기인。결과 miR-34a-5p재K562세포홍계분화과정중정현표체하강추세；재K562세포중과표체 miR-34a-5p 가억제 hemin 유도적홍계분화（ P ＜0.05）；반지，간우K562내원적miR-34a-5p표체회대K562홍계분화산생촉진작용（ P＜0.01）；령일방면，miR-34a-5p통과파향억제c-MYB적표체억제세포향홍계분화。결론 miR-34a-5p통과억제c-MYB재K562세포조기홍계분화과정중발휘촉진작용。
Objective To study the effects of microRNA-34a-5p on erythroid differentiation of K562 cells.Methods K562 cells were transfected with the microRNA-34a-5p mimics and antisense inhibitors specifically targeting mi-croRNA-34a-5p, respectively.The effects of over-expression or knocking-down of microRNA-34a-5p were exam-ined by Quantitative RT-PCR.Flow cytometry was performed to detect specific surface marker of erythroid cells . The benzidine staining assay was used to access the differentiation of K 562 cells.Western blot was performed to de-tect miRNA targets.Results microRNA-34a-5p was down-regulated at the early stage of K562 erythroid differenti-ation.Over-expression of microRNA-34a-5p in K562 cells attenuates erythroid differentiation , in contrast, inhibi-tion of microRNA-34a-5p accelerates erythroid pheotypes in K562 cells.c-MYB was found to be the direct target of microRNA-34 a-5 p in erythroid cells .Conclusions microRNA-34 a-5 p regulates early erythroid differentiation of K562 cells via repressing c-MYB.