CAJ | 학술논문

目的：观察茵陈蒿汤对酒精性肝病大鼠肝脏及小肠组织病理的改善作用。方法采用“白酒-吡唑-植物油”混合灌胃法制备大鼠酒精性肝病模型,成模后分别以茵陈蒿汤与双歧杆菌三联活菌胶囊(商品名：培菲康)灌胃治疗2周,观察肝与小肠组织病理学改变,测定各组大鼠血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase, AST)、谷氨酰转肽酶(glutamyltranspetidase,GGT)活性及白蛋白(albumin,ALB)浓度,测定各组大鼠肝组织羟脯氨酸(hydroxyproline,Hyp)含量。结果与空白组比较,模型大鼠肝组织出现显著的肝纤维化及肝细胞脂肪变性,小肠绒毛出现明显缺损,腺体萎缩,伴炎细胞浸润,模型大鼠肝组织 Hyp含量及血清 GGT 活性显著高于空白组(P<0.01),血清 ALB 浓度显著低于空白组(P<0.01),但血清ALT 及 AST 活性与空白组无显著差异(P>0.05)；与模型组比较,茵陈蒿汤组大鼠肝纤维化及肝细胞脂肪变性明显减轻,小肠绒毛较完整,腺体排列较规则,肝组织 Hyp 含量及血清 GGT 活性显著降低(P<0.05或 P<0.01)；双歧杆菌组大鼠小肠黏膜损伤显著减轻,但肝组织病理改善不明显,肝组织 Hyp 含量与模型大鼠无显著差异(P>0.05)。结论茵陈蒿汤能够有效减轻酒精性肝病大鼠肝纤维化与脂肪变性,同时显著改善小肠黏膜损伤。
목적：관찰인진호탕대주정성간병대서간장급소장조직병리적개선작용。방법채용“백주-필서-식물유”혼합관위법제비대서주정성간병모형,성모후분별이인진호탕여쌍기간균삼련활균효낭(상품명：배비강)관위치료2주,관찰간여소장조직병이학개변,측정각조대서혈청병안산안기전이매(alanine aminotransferase,ALT)、천동안산안기전이매(aspartate aminotransferase, AST)、곡안선전태매(glutamyltranspetidase,GGT)활성급백단백(albumin,ALB)농도,측정각조대서간조직간포안산(hydroxyproline,Hyp)함량。결과여공백조비교,모형대서간조직출현현저적간섬유화급간세포지방변성,소장융모출현명현결손,선체위축,반염세포침윤,모형대서간조직 Hyp함량급혈청 GGT 활성현저고우공백조(P<0.01),혈청 ALB 농도현저저우공백조(P<0.01),단혈청ALT 급 AST 활성여공백조무현저차이(P>0.05)；여모형조비교,인진호탕조대서간섬유화급간세포지방변성명현감경,소장융모교완정,선체배렬교규칙,간조직 Hyp 함량급혈청 GGT 활성현저강저(P<0.05혹 P<0.01)；쌍기간균조대서소장점막손상현저감경,단간조직병리개선불명현,간조직 Hyp 함량여모형대서무현저차이(P>0.05)。결론인진호탕능구유효감경주정성간병대서간섬유화여지방변성,동시현저개선소장점막손상。
Objective To investigate the effect of Yinchenhao decoction on both hepatic and intestinal histopathology in rats with alcoholic liver disease. Methods Rats model with alcoholic liver disease was established by “Liquor - pyrazole - vegetable oil” mixture gavaging for 12 weeks. After 2 weeks treatment with either Yinchenhao decoction or Bifidobacterium,Lactobacillus and Enterococcus Capsules (Bilico),histopathological observation of hepatic and intestinal lesions were conducted by microscopy and serum activities of Alanine aminotransferase ( ALT ), Aspartate aminotransferase ( AST ) , Glutamyltranspetidase ( GGT) , ALB concentration and hepatic hydroxyproline ( Hyp) content were detected. Results Compared to the control group,the model rats showed significant hepatic fibrosis and steatosis accompanied by shorter and sparsely arranged intestinal villi,glandular atrophy and inflammatory cell infiltration. The content of Hyp and activity of serum GGT of model rats was significantly higher than that of the control group (P<0. 01),while the concentration of serum ALB was significantly lower than the control group (P<0. 01). However,there was no significant difference in serum ALT and AST activity between the control group and the model group (P>0. 05). Compared to the model group, hepatic fibrosis and steatosis in Yinchenhao decoction-treated group were significantly reduced with more complete intestinal villi,more regular arranged submucosal glands as well as significantly decreased content of hepatic Hyp and serum GGT activity ( P < 0. 05, P < 0. 01). Bilico-treated groups showed obvious improvement only in intestinal mucosal injuries, not in hepatic histopathology and Hyp content. Conclusions Yinchenhao decoction can effectively alleviate hepatic fibrosis and steatosis as well as the intestinal mucosal injury in rats with alcoholic liver disease.