CAJ | 학술논문

目的：通过 DNA 条形码—产地—形态分析联用的方法对巴西马钱属植物药 QUINA进行基原鉴定研究,为这种药材的开发利用提供依据。方法通过提取样品 DNA、PCR 扩增及双向测序,获得样品的内转录间隔区(internal transcribed spacer,ITS)序列,利用 NCBI 数据库中的 BLAST功能计算相似度,对其基原进行 DNA 条形码鉴定；根据其产地信息对 DNA 条形码鉴定结果进行筛选和分析；依据其形态特征验证鉴定结果。结果本次收集的植物类药材 QUINA 来源于马钱科马钱属植物 Strychnos pseudo quina A. St.-Hil.的根皮。结论 DNA 条形码—产地—形态三者联用的方法可弥补单一鉴定方法的不足,能够快速、准确的对未知样品进行基原鉴定。
목적：통과 DNA 조형마—산지—형태분석련용적방법대파서마전속식물약 QUINA진행기원감정연구,위저충약재적개발이용제공의거。방법통과제취양품 DNA、PCR 확증급쌍향측서,획득양품적내전록간격구(internal transcribed spacer,ITS)서렬,이용 NCBI 수거고중적 BLAST공능계산상사도,대기기원진행 DNA 조형마감정；근거기산지신식대 DNA 조형마감정결과진행사선화분석；의거기형태특정험증감정결과。결과본차수집적식물류약재 QUINA 래원우마전과마전속식물 Strychnos pseudo quina A. St.-Hil.적근피。결론 DNA 조형마—산지—형태삼자련용적방법가미보단일감정방법적불족,능구쾌속、준학적대미지양품진행기원감정。
Objective To study the variety identification of QUINA of Strychnos Linn. with the combined analysis of DNA barcoding-origin-morphology and provide an evidence for the exploitation of this kind of herbal in Brazil. Methods ITS sequences were amplified by PCR from genomic DNA and sequenced. , BLAST( basic local alignment search tool) in NCBI database was used to calculate the similarity of sequences to identify the variety of samples; then the DNA barcoding identification results were screened and analyzed in reference to its origin information; finally, the morphological characteristics of samples was used to verify the accuracy of identification. Results QUINA collected from market in Brazil was the root bark of Strychnos pseudo quina A. St. -Hil. Conclusion The combined analysis of DNA barcoding-origin-morphology could make up for the deficit of onefold identification method. It contributes to identifying the variety of unidentified samples quickly and accurately.