环球中医药
環毬中醫藥
배구중의약
Global Traditional Chinese Medicine
2015年
11期
1306-1308
,共3页
臧艺玫%顾选%余春霞%王娟%韩丽%吴浩忠%肖瑶%赵百孝%刘春生%马条华
臧藝玫%顧選%餘春霞%王娟%韓麗%吳浩忠%肖瑤%趙百孝%劉春生%馬條華
장예매%고선%여춘하%왕연%한려%오호충%초요%조백효%류춘생%마조화
柑橘属%药材粉末%基原鉴定%DNA 条形码
柑橘屬%藥材粉末%基原鑒定%DNA 條形碼
감귤속%약재분말%기원감정%DNA 조형마
Citrus L.%Powder%Varieties identification%DNA barcoding
目的：利用显微-DNA 条形码-产地分析联用的方法对巴西柑橘属植物药 FARINHA DE LARANJA 粉末进行基原鉴定研究。方法应用显微鉴定的方法对药材粉末的基原进行初步判定；再提取样品 DNA,扩增 ITS 序列,利用 NCBI 数据库中的 BLAST 功能分析相似度,对样品品种进行DNA 条形码鉴定；最后结合产地信息对数据库中的序列进行筛选。结果显微-DNA 条形码-产地综合分析结果表明,该样品为芸香科柑橘属 Citrus L.植物酸橙 Citrus aurantium 的干燥未成熟果实粉末。结论巴西植物药 FARINHA DE LARANJA 的鉴定可为扩大枳实、枳壳等中药材的药用资源提供依据。
目的：利用顯微-DNA 條形碼-產地分析聯用的方法對巴西柑橘屬植物藥 FARINHA DE LARANJA 粉末進行基原鑒定研究。方法應用顯微鑒定的方法對藥材粉末的基原進行初步判定；再提取樣品 DNA,擴增 ITS 序列,利用 NCBI 數據庫中的 BLAST 功能分析相似度,對樣品品種進行DNA 條形碼鑒定；最後結閤產地信息對數據庫中的序列進行篩選。結果顯微-DNA 條形碼-產地綜閤分析結果錶明,該樣品為蕓香科柑橘屬 Citrus L.植物痠橙 Citrus aurantium 的榦燥未成熟果實粉末。結論巴西植物藥 FARINHA DE LARANJA 的鑒定可為擴大枳實、枳殼等中藥材的藥用資源提供依據。
목적：이용현미-DNA 조형마-산지분석련용적방법대파서감귤속식물약 FARINHA DE LARANJA 분말진행기원감정연구。방법응용현미감정적방법대약재분말적기원진행초보판정；재제취양품 DNA,확증 ITS 서렬,이용 NCBI 수거고중적 BLAST 공능분석상사도,대양품품충진행DNA 조형마감정；최후결합산지신식대수거고중적서렬진행사선。결과현미-DNA 조형마-산지종합분석결과표명,해양품위예향과감귤속 Citrus L.식물산등 Citrus aurantium 적간조미성숙과실분말。결론파서식물약 FARINHA DE LARANJA 적감정가위확대지실、지각등중약재적약용자원제공의거。
Objective o study the variety identification of a kind of herbal powder of FARINHA DE LARANJA of Citrus L. in Brazil with a combaned analysis of microscope - DNA barcoding - origin. Methods Microscopic identification was used to identify the variety of samples preliminarily; then ITS se-quences were amplified by PCR and sequenced, and BLAST in NCBI database was used to calculate the similarity of sequences to identify variety of samples. , Finally, the DNA barcoding identification results were screened and analyzed according to its geographical origin information. Results The combined analysis of microscopic - DNA barcoding - origin indicated that FARINHA DE LARANJA collected from market in Brazil was the dried fruit powder of Citrus aurantium. Conclusion The identification of Brazil FARINHA DE LARANJA could enrich the alternative resources of Zhiqiao and Zhishi.
