CAJ | 학술논문

目的：对巴西草药番泻叶 SENE 进行品种鉴别，进而研究其中番泻苷 A 和番泻苷 B的含量并与中国番泻叶番泻苷 A、B 的比较，为其品种整理、临床使用及资源开发提供参考。方法利用 DNA 条形码—产地—形态分析联用技术鉴定巴西草药番泻叶 SENE 的基原，高效液相色谱技术测定番泻苷 A 和番泻苷 B 的含量。结果品种鉴定结果为番泻叶，其中番泻苷 A 和番泻苷 B 两者的平均总含量为巴西番泻叶(1.56%)>中国番泻叶(1.52%)>2010年版《中华人民共和国药典》(一部)的含量要求(1.10%)。结论巴西草药番泻叶来源于番泻叶 Senna alexandrina Mill.，其番泻苷 A 和番泻苷 B 的含量与中国番泻叶差异不显著，且能达到药典要求。
목적：대파서초약번사협 SENE 진행품충감별，진이연구기중번사감 A 화번사감 B적함량병여중국번사협번사감 A、B 적비교，위기품충정리、림상사용급자원개발제공삼고。방법이용 DNA 조형마—산지—형태분석련용기술감정파서초약번사협 SENE 적기원，고효액상색보기술측정번사감 A 화번사감 B 적함량。결과품충감정결과위번사협，기중번사감 A 화번사감 B 량자적평균총함량위파서번사협(1.56%)>중국번사협(1.52%)>2010년판《중화인민공화국약전》(일부)적함량요구(1.10%)。결론파서초약번사협래원우번사협 Senna alexandrina Mill.，기번사감 A 화번사감 B 적함량여중국번사협차이불현저，차능체도약전요구。
Objective To identify the species of Brazilian herb SENE and study on the content of sennoside A and sennoside B, then to compare the results with Chinese Sennae folium, and provide reference for varieties systematics, clinical use and resource exploitation. Methods The source of varieties of Brazilian herb SENE was identified by a technique combined DNA barcoding, geographical origin and morphological features, and the content of sennoside A and sennoside B was analyzed by HPLC. Results DNA barcoding identification indicated that SENE was Senna alexandrina, and its average total content of sennoside A and sennoside B (1. 56% ) was higher than its Chinese counterpart (1. 52% ) and also higher than what is required in Chinese Pharmacopoeia (1. 10% ). Conclusion Brazilian herb SENE derives from Senna alexandrina Mill. , and its sennoside A and sennoside B content have no significant difference with its Chinese counterpart which meets pharmacopoeia requirement.