CAJ | 학술논문

目的：对巴西草药牛蒡的品种进行鉴定，同时研究其中的牛蒡苷含量，为其品种整理和药理研究提供依据。方法利用 DNA 条形码—产地—形态分析联用技术鉴定巴西草药牛蒡的基原，高效液相色谱法测定其牛蒡苷含量。结果 DNA 条形码初步鉴定巴西草药牛蒡为菊科牛蒡属植物，且与牛蒡达到最高相似度99%，产地及形态分析进一步确证其为牛蒡的地上部分；巴西牛蒡草中牛蒡苷平均含量为0.281%，明显低于中国牛蒡草牛蒡苷平均含量3.72%。结论巴西草药牛蒡来源于牛蒡 Arctium lappa L.的干燥地上部分，其牛蒡苷含量极低。
목적：대파서초약우방적품충진행감정，동시연구기중적우방감함량，위기품충정리화약리연구제공의거。방법이용 DNA 조형마—산지—형태분석련용기술감정파서초약우방적기원，고효액상색보법측정기우방감함량。결과 DNA 조형마초보감정파서초약우방위국과우방속식물，차여우방체도최고상사도99%，산지급형태분석진일보학증기위우방적지상부분；파서우방초중우방감평균함량위0.281%，명현저우중국우방초우방감평균함량3.72%。결론파서초약우방래원우우방 Arctium lappa L.적간조지상부분，기우방감함량겁저。
Objective To trace the source and study on the content of arctiin of Brazilian herb BARDANA for varieties systematics and pharmacological analysis. Methods The source of varieties of Brazilian herb BARDANA was identified by a technology combined DNA barcoding, plant geographical origin and morphological features , and the content of arctiin was analyzed by HPLC. Results DNA barcoding preliminarily identified BARDANA belongs to the stretch of Arctium of Compositae with a similarity level of 99% . Geographical and morphological verification further confirmed the aforementioned finding. The content of arctiin in the Brazilian was 0. 281% ,which was significantly lower than the Chinese arctiin (3. 72% ). Conclusion Brazilian herb BARDANA derives from Arctium lappa L. , and the content of arctiin was very low.