环球中医药
環毬中醫藥
배구중의약
Global Traditional Chinese Medicine
2015年
11期
1291-1294
,共4页
顾选%崔秀梅%余春霞%马长华%肖瑶%韩丽%刘春生%赵百孝%哈略
顧選%崔秀梅%餘春霞%馬長華%肖瑤%韓麗%劉春生%趙百孝%哈略
고선%최수매%여춘하%마장화%초요%한려%류춘생%조백효%합략
巴西草药%药用蒲公英%混伪品%DNA 条形码%鉴定
巴西草藥%藥用蒲公英%混偽品%DNA 條形碼%鑒定
파서초약%약용포공영%혼위품%DNA 조형마%감정
Brazilian herb%DENTE DE LE?O%Adulterants%DNA barcoding%Identifica-tion
目的：对巴西市场上销售的药用蒲公英的混伪品进行鉴定及生药学研究。方法通过提取样品 DNA、PCR 扩增及双向测序，获得样品的 ITS 序列。采用相似度分析法进行分子鉴定；根据植物产地分布特征和形态特征验证结果，三种方法联用获得鉴定结果；分析混伪品的性状和显微特征。结果 DNA 条形码研究表明巴西药用蒲公英的混伪品来源于菊科植物假蒲公英猫儿菊Hypochaeris radicata 的全草，产地分析和形态分析验证了 DNA 条形码鉴定结果。结论巴西药用蒲公英的混伪品来源于菊科蒲公英属植物假蒲公英猫儿菊 Hypochaeris radicata 的全草。混伪品的生药学特征能够为巴西草药 DENTE DE LE?O 的真伪鉴别提供依据。
目的：對巴西市場上銷售的藥用蒲公英的混偽品進行鑒定及生藥學研究。方法通過提取樣品 DNA、PCR 擴增及雙嚮測序，穫得樣品的 ITS 序列。採用相似度分析法進行分子鑒定；根據植物產地分佈特徵和形態特徵驗證結果，三種方法聯用穫得鑒定結果；分析混偽品的性狀和顯微特徵。結果 DNA 條形碼研究錶明巴西藥用蒲公英的混偽品來源于菊科植物假蒲公英貓兒菊Hypochaeris radicata 的全草，產地分析和形態分析驗證瞭 DNA 條形碼鑒定結果。結論巴西藥用蒲公英的混偽品來源于菊科蒲公英屬植物假蒲公英貓兒菊 Hypochaeris radicata 的全草。混偽品的生藥學特徵能夠為巴西草藥 DENTE DE LE?O 的真偽鑒彆提供依據。
목적：대파서시장상소수적약용포공영적혼위품진행감정급생약학연구。방법통과제취양품 DNA、PCR 확증급쌍향측서，획득양품적 ITS 서렬。채용상사도분석법진행분자감정；근거식물산지분포특정화형태특정험증결과，삼충방법련용획득감정결과；분석혼위품적성상화현미특정。결과 DNA 조형마연구표명파서약용포공영적혼위품래원우국과식물가포공영묘인국Hypochaeris radicata 적전초，산지분석화형태분석험증료 DNA 조형마감정결과。결론파서약용포공영적혼위품래원우국과포공영속식물가포공영묘인국 Hypochaeris radicata 적전초。혼위품적생약학특정능구위파서초약 DENTE DE LE?O 적진위감별제공의거。
Objective To study a new adulterant of DENTE DE LE?O in Brazil and perform a pharmaceutical analysi. Methods Total genomic DNA was isolated from the materials,,and nuclear DNA ITS sequences were amplified and sequenced. Similarity identification of BLAST analysis was performed. Next, the results were verified according to plant geographical distribution and morphological profiles. Finally, the features of adulterant were demonstrated through microscopic observation. Results Adulterants of Brazil herbs DENTE DE LE?O was Hypochaeris radicata Linnaeus, Sp. Pl. The origin analysis and morphological analysis verified the results of DNA barcoding. Conclusion Adulterants of Brazilian herb DENTE DE LE?O are from the Asteraceae plant Hypochaeris radicata with the whole plants. Adulterants pharmacognosy features can provide the basis for identification of Brazil herbs DENTE DE LE?O .
