CAJ | 학술논문

目的：研究小鼠内外毛细胞胞吞功能的异同，探讨毛细胞胞吞功能与动物听功能之间的关系。方法选择出生后1月龄的正常C57BL/6J小鼠耳蜗基底膜在体外培养，以染料FM1-43为胞吞示踪剂，应用活细胞成像技术观察耳蜗内外毛细胞胞吞现象。结果内毛细胞的胞吞活动主要集中在细胞底部及核下区，而外毛细胞的胞吞活动则主要集中在核上区和外侧壁区。而且，在不同的观察时间点上，内毛细胞对FM1-43的摄入量均显著高于外毛细胞(P<0.05)。结论内毛细胞的胞吞活动集中出现在细胞底部及核下区，说明这种胞吞活动与内毛细胞带状突触的功能密切相关；外毛细胞的胞吞活动主要出现在核上区及细胞外侧壁，表明这种活动更多参与了外毛细胞纤毛及离子通道。内毛细胞比外毛细胞具有更强大的胞吞功能，表明内毛细胞在听功能的发育和维持中发挥着更为关键的作用。
목적：연구소서내외모세포포탄공능적이동，탐토모세포포탄공능여동물은공능지간적관계。방법선택출생후1월령적정상C57BL/6J소서이와기저막재체외배양，이염료FM1-43위포탄시종제，응용활세포성상기술관찰이와내외모세포포탄현상。결과내모세포적포탄활동주요집중재세포저부급핵하구，이외모세포적포탄활동칙주요집중재핵상구화외측벽구。이차，재불동적관찰시간점상，내모세포대FM1-43적섭입량균현저고우외모세포(P<0.05)。결론내모세포적포탄활동집중출현재세포저부급핵하구，설명저충포탄활동여내모세포대상돌촉적공능밀절상관；외모세포적포탄활동주요출현재핵상구급세포외측벽，표명저충활동경다삼여료외모세포섬모급리자통도。내모세포비외모세포구유경강대적포탄공능，표명내모세포재은공능적발육화유지중발휘착경위관건적작용。
Objective To report different patterns of endocytosis in murine cochlear inner and outer hair cells (IHCs&OHCs). Methods Normal C57BL/6J mice (1 month old) were used to observe different patterns of endocytosis in IHCs and OHCs in vitro using live cell imaging of FM1-43. Results Intense fluorescence den-sity of FM1-43 indicating massive endocytosis activities was identified at the base and blow the nucleus in IHCs. In contrast, major endocytosis activities in OHCs were found at or near the lateral cellular wall and above the nucleus. FM1-43 uptake in IHCs was higher than that in OHCs (P<0.05). Conclusion Active endocy-tosis at or near the basolateral area of the IHC suggests that endocytosis in IHCs may involve ribbon synapse plasticity. Endocytosis activities near the lateral wall and in the supranuclear area in OHC indicate that endocy-tosis in OHC involves tip-links or iron channels. Higher levels of FM1-43 uptake by IHCs may indicate IHC’s key roles in hearing development and maintenance.