CAJ | 학술논문

目的：研究裸花紫珠提取物凝血作用及其有效部位。方法以质控血浆为研究对象,通过测定裸花紫珠粗提物及各分离部位和单体对凝血酶原时间(prothrombin time,PT)、凝血酶时间(thrombin time,TT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)、血浆纤维蛋白原(fibrinogen,FIB)四项指标观察其对凝血系统的影响。结果与溶媒对照比较,裸花紫珠粗提物能明显缩短 PT、APTT、TT 的时间,能明显提高 FIB 含量；裸花紫珠40%甲醇洗脱部位能明显缩短 APTT、TT 的时间,能明显提高 FIB 含量；与粗提物比较,40%甲醇洗脱部位效果最接近于粗提物。结论裸花紫珠有较好的凝血效果,其有效部位可能在40%甲醇洗脱部位。
목적：연구라화자주제취물응혈작용급기유효부위。방법이질공혈장위연구대상,통과측정라화자주조제물급각분리부위화단체대응혈매원시간(prothrombin time,PT)、응혈매시간(thrombin time,TT)、활화부분응혈활매시간(activated partial thromboplastin time,APTT)、혈장섬유단백원(fibrinogen,FIB)사항지표관찰기대응혈계통적영향。결과여용매대조비교,라화자주조제물능명현축단 PT、APTT、TT 적시간,능명현제고 FIB 함량；라화자주40%갑순세탈부위능명현축단 APTT、TT 적시간,능명현제고 FIB 함량；여조제물비교,40%갑순세탈부위효과최접근우조제물。결론라화자주유교호적응혈효과,기유효부위가능재40%갑순세탈부위。
Objective To study the effect of active fraction from Callicarpa nudiflora on blood co-agulation system. Methods The effect of active fraction from Callicarpa nudiflora on blood coagulation system were observed with prothrombin time(PT), activated partial thromboplastin time(APTT), thrombin time(TT) and fibrinogen(FIB) of the constituents blood samples were tested. Results Compared with the blank control, Callicarpa nudiflora extract could significantly lessen PT, TT, APTT, and could obviously increased the content of FIB; 40% Methanol elution par could significantly lessen TT, APTT, and could obviously increased the content of FIB. Conclusion The hemostatic effect of active fraction from Callicarpa nudiflora may be 40% Methanol elution par of Callicarpa nudiflora.