环球中医药
環毬中醫藥
배구중의약
Global Traditional Chinese Medicine
2015年
11期
1324-1328
,共5页
邹大威%高彦彬%刘迎新%耿建国%彭麒朕%龚慕辛%朱智耀%李娇阳%杨鑫伟%王晓磊
鄒大威%高彥彬%劉迎新%耿建國%彭麒朕%龔慕辛%硃智耀%李嬌暘%楊鑫偉%王曉磊
추대위%고언빈%류영신%경건국%팽기짐%공모신%주지요%리교양%양흠위%왕효뢰
糖尿病肾病%糖肾宁%KK-Ay 小鼠%基质金属蛋白酶-9%基质金属蛋白酶组织抑制剂-1
糖尿病腎病%糖腎寧%KK-Ay 小鼠%基質金屬蛋白酶-9%基質金屬蛋白酶組織抑製劑-1
당뇨병신병%당신저%KK-Ay 소서%기질금속단백매-9%기질금속단백매조직억제제-1
Diabetic nephropathy%Tangshenning%KK-Ay mice%Matrix metalloproteinase-9%Tissue inhibitor of metalloproteinase 1
目的:观察糖肾宁对自发性2型糖尿病 KK-Ay 小鼠肾功能及肾组织降解酶系基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)、基质金属蛋白酶组织抑制剂-1( tissue inhibitor of metalloproteinase 1,TIMP-1)蛋白及基因表达的影响。方法采用自发性2型糖尿病 KK-Ay 小鼠建立糖尿病肾病模型,随机分为模型组、缬沙坦组和糖肾宁组各20只,设立 C57BL/6J 小鼠20只为空白组;予通心络及缬沙坦干预12周后,测定各组小鼠血清尿素氮(blood urea nitrogen,BUN)、血清肌酐(serum creatinine,SCr),采用 Western Blot 、Realtime-PCR、免疫组化方法检测 MMP-9、TIMP-1蛋白表达或定位及基因表达水平。结果与空白组比较,模型组小鼠 BUN、SCr 均明显升高(P<0.05);肾组织 TIMP-1蛋白及基因表达明显升高、MMP-9蛋白及基因表达明显降低(P<0.05);与模型组比较,糖肾宁组和缬沙坦组 BUN、SCr 均明显降低(P<0.05),肾组织 TIMP-1蛋白及基因表达明显降低、MMP-9蛋白及基因表达明显升高(P<0.05)。结论糖肾宁能够保护自发性2型糖尿病 KK-Ay小鼠肾功能、延缓糖尿病肾病肾纤维化进展,调控糖尿病肾病肾组织降解酶系可能是糖肾宁防治糖尿病肾病的作用机制之一。
目的:觀察糖腎寧對自髮性2型糖尿病 KK-Ay 小鼠腎功能及腎組織降解酶繫基質金屬蛋白酶-9(matrix metalloproteinase-9,MMP-9)、基質金屬蛋白酶組織抑製劑-1( tissue inhibitor of metalloproteinase 1,TIMP-1)蛋白及基因錶達的影響。方法採用自髮性2型糖尿病 KK-Ay 小鼠建立糖尿病腎病模型,隨機分為模型組、纈沙坦組和糖腎寧組各20隻,設立 C57BL/6J 小鼠20隻為空白組;予通心絡及纈沙坦榦預12週後,測定各組小鼠血清尿素氮(blood urea nitrogen,BUN)、血清肌酐(serum creatinine,SCr),採用 Western Blot 、Realtime-PCR、免疫組化方法檢測 MMP-9、TIMP-1蛋白錶達或定位及基因錶達水平。結果與空白組比較,模型組小鼠 BUN、SCr 均明顯升高(P<0.05);腎組織 TIMP-1蛋白及基因錶達明顯升高、MMP-9蛋白及基因錶達明顯降低(P<0.05);與模型組比較,糖腎寧組和纈沙坦組 BUN、SCr 均明顯降低(P<0.05),腎組織 TIMP-1蛋白及基因錶達明顯降低、MMP-9蛋白及基因錶達明顯升高(P<0.05)。結論糖腎寧能夠保護自髮性2型糖尿病 KK-Ay小鼠腎功能、延緩糖尿病腎病腎纖維化進展,調控糖尿病腎病腎組織降解酶繫可能是糖腎寧防治糖尿病腎病的作用機製之一。
목적:관찰당신저대자발성2형당뇨병 KK-Ay 소서신공능급신조직강해매계기질금속단백매-9(matrix metalloproteinase-9,MMP-9)、기질금속단백매조직억제제-1( tissue inhibitor of metalloproteinase 1,TIMP-1)단백급기인표체적영향。방법채용자발성2형당뇨병 KK-Ay 소서건립당뇨병신병모형,수궤분위모형조、힐사탄조화당신저조각20지,설립 C57BL/6J 소서20지위공백조;여통심락급힐사탄간예12주후,측정각조소서혈청뇨소담(blood urea nitrogen,BUN)、혈청기항(serum creatinine,SCr),채용 Western Blot 、Realtime-PCR、면역조화방법검측 MMP-9、TIMP-1단백표체혹정위급기인표체수평。결과여공백조비교,모형조소서 BUN、SCr 균명현승고(P<0.05);신조직 TIMP-1단백급기인표체명현승고、MMP-9단백급기인표체명현강저(P<0.05);여모형조비교,당신저조화힐사탄조 BUN、SCr 균명현강저(P<0.05),신조직 TIMP-1단백급기인표체명현강저、MMP-9단백급기인표체명현승고(P<0.05)。결론당신저능구보호자발성2형당뇨병 KK-Ay소서신공능、연완당뇨병신병신섬유화진전,조공당뇨병신병신조직강해매계가능시당신저방치당뇨병신병적작용궤제지일。
[Abstract ]Objective To explore effects of Tangshenning on expression of matrix metalloproteinase-9 (MMP-9)、tissue inhibitor of metalloproteinase 1 ( TIMP-1) in diabetic nephropathy mice model. Methods Diabetic male KK-Ay mice were randomLy divided into three groups: the model group (n= 20),the valsartan group( n = 20) and the Tangshenning group ( n = 20). Male C57BL / 6J (n=20)mice were designed as the control group. After intervention with Tangshenning and valsartan for 12 weeks,serum creatinine (Scr) and serum urea nitrogen(BUN) were examined. Expression of MMP-9, TIMP-1 were accessed by Realtime-Pcr , Western Blot and Immunohistochemistry. Results Compared with control group, BUN and SCr were significantly increased (P<0. 05), while them of mice in valsartan and tangshenning group were decreased compared with model group(P<0. 05). Compared with the control group, the protein and gene expression of MMP9 and TIMP-1 both have the same trend in model group, the expression of MMP9 were decreased significantly and the expression of TIMP-1 were increased significantly (P<0. 05). Through the treatment of Tangshenning and valsartan for 12 weeks, the expression of TIMP-1 were down-regulated and the expression of MMP-9 were up-regulated in diabeticKK-Ay mice(P<0. 05). Conclusion Tangshenning could protect renal function and delay the process of renal fibrosis partly through regulating extracellular matrix degradation enzymes MMP-9 / TIMP-1 expression.