CAJ | 학술논문

目的：利用性状—显微—DNA 条形码联用的方法对巴西牛至属植物药 HORTELA 进行基原鉴定研究。方法应用性状鉴定的方法初步对药材进行真伪鉴别；再应用显微鉴定的方法验证性状鉴定结果；最后提取样品 DNA,扩增 ITS 序列,利用 NCBI 数据库中的 BLAST 功能分析相似度,对样品品种进行 DNA 条形码鉴定。结果性状—显微—DNA 条形码综合分析结果表明,该样品为牛至属植物 Origanum elongatum(Bonnet) Emb.& Maire.的干燥地上部分。结论 HORTELA 可能为多基原植物类药材,其来源为牛至属 Origanum elongatum (Bonnet) Emb.& Maire.和薄荷属Mentha rotundifolia (Linn.) Huds.干燥地上部分。
목적：이용성상—현미—DNA 조형마련용적방법대파서우지속식물약 HORTELA 진행기원감정연구。방법응용성상감정적방법초보대약재진행진위감별；재응용현미감정적방법험증성상감정결과；최후제취양품 DNA,확증 ITS 서렬,이용 NCBI 수거고중적 BLAST 공능분석상사도,대양품품충진행 DNA 조형마감정。결과성상—현미—DNA 조형마종합분석결과표명,해양품위우지속식물 Origanum elongatum(Bonnet) Emb.& Maire.적간조지상부분。결론 HORTELA 가능위다기원식물류약재,기래원위우지속 Origanum elongatum (Bonnet) Emb.& Maire.화박하속Mentha rotundifolia (Linn.) Huds.간조지상부분。
Objective To study the variety identification of HORTELA of Origanum Linn. in Brazil with a combined analysis of macroscopic-microscopic-DNA barcoding. Methods Macroscopic iden-tification was used to identify the authenticity of samples preliminarily; then the results was verified by mi-croscopic identification; finally, ITS sequences were amplified by PCR and sequenced, and BLAST in NCBI ( basic local alignment search tool) database was used to calculate the similarity of sequences to identify variety of samples. Results The combined analysis of macroscopic-microscopic-DNA barcoding indicated that Hortela collected from market in Brazil is the dried overground part of Origanum elongatum (Bonnet) Emb. & Maire. Conclusion HORTELA may be a multi-source herb ,which originates from the dried overground part of Origanum elongatum(Bonnet) Emb. & Maire and Mentha rotundifolia (Linn. ) Huds.