CAJ | 학술논문

目的：对巴西草药 CENTELHA ASIáTICA 进行生药学研究。方法采用 DNA 条形码技术对巴西草药的叶片提取 DNA、扩增 ITS 序列、双向测序，采用相似度法进行物种鉴定，再结合产地信息和性状特征核对 DNA 条形码鉴定结果进行验证。结果巴西草药 CENTELHA ASIáTICA 与NCBI 核酸数据库积雪草 ITS 序列达到最高相似度，相似度为99%，鉴定其为伞形科植物积雪草Centella asiatica(L.) Urban。在地理分布上，巴西草药 CENTELHA ASIáTICA 符合积雪草的分布特征；从形态分析上，样品与积雪草特征相符合。结论基于 DNA 条形码-产地-形态分析的鉴定方法能够对巴西草药 CENTELHA ASIáTICA 进行鉴定，鉴定该物种来源于伞形科植物积雪草 Centella asiatica(L.) Urban 的干燥全草。生药学研究能够为 CENTELHA ASIáTICA 品种鉴定及质量标准研究提供依据。
목적：대파서초약 CENTELHA ASIáTICA 진행생약학연구。방법채용 DNA 조형마기술대파서초약적협편제취 DNA、확증 ITS 서렬、쌍향측서，채용상사도법진행물충감정，재결합산지신식화성상특정핵대 DNA 조형마감정결과진행험증。결과파서초약 CENTELHA ASIáTICA 여NCBI 핵산수거고적설초 ITS 서렬체도최고상사도，상사도위99%，감정기위산형과식물적설초Centella asiatica(L.) Urban。재지리분포상，파서초약 CENTELHA ASIáTICA 부합적설초적분포특정；종형태분석상，양품여적설초특정상부합。결론기우 DNA 조형마-산지-형태분석적감정방법능구대파서초약 CENTELHA ASIáTICA 진행감정，감정해물충래원우산형과식물적설초 Centella asiatica(L.) Urban 적간조전초。생약학연구능구위 CENTELHA ASIáTICA 품충감정급질량표준연구제공의거。
Objective To study Brazilian herbal CENTELHA ASIáTICA from the perspective of bi-ological pharmacy. Methods By applying DNA barcoding technology, total genomic DNA was isolated from the materials, and nuclear DNA ITS sequences were amplified and sequenced;DNA fragments were collated and matched by using ContingExpress. Similarity identification of BLAST analysis was performed. Next, the DNA bar code was verified by the information of plant origin and morphology. Families and genera were identified by Molecular identification. Results ITS sequence of Brazil herb CENTELHA ASIáTICA and Centella asiatica (L. ) Urban was 99% identical. The results can be solidified by their sim-ilarities in geographical distribution and morphological features. Conclusion Based on DNA barcoding-origin-morphological analysis Brazilian herbal CENTELHA ASIáTICA was identified as Centella asiatica L. and biological pharmacy can provide the basis for the identification and quality standard of CENTELHA ASIáTICA varieties.