CAJ | 학술논문

为探究盐度对虎斑乌贼(Sepia pharaonis)幼体生长和酶活的影响，采用突变和渐变单因子试验方法研究了不同盐度(18‰、21‰、24‰、27‰、30‰、33‰)条件对其存活率、特定生长率、增重率、肝体指数和超氧化物歧化酶(SOD)、碱性磷酸酶(AKP)、谷丙转氨酶(GPT)、谷草转氨酶(GOT)等肝脏代谢酶酶活的影响。结果表明：经21d培养，盐度突变对幼体存活率和行为有显著影响(p<0.05)，盐度24‰组幼体存活率最高(77.78%±6.94%)，盐度27‰组特定生长率(7.29%×d-1±0.27%×d-1)和增重率(198.42%±12.36%)最大，肝体比随着盐度的上升呈现上升趋势，以盐度30‰最高；盐度渐变对幼体存活率、特定生长率、增重率、肝体比和酶活影响显著(p<0.05)。盐度24‰组存活率(87.78%±1.92%)最高，盐度27‰时特定生长率(6.88%×d-1±0.13%×d-1)和增重率(180.96%±5.13%)最大，肝体比在盐度30‰时达到最大。SOD酶活随着盐度的上升呈现先下降后上升的趋势，盐度27‰最低，而AKP、GPT和GOT酶活呈现先上升后下降的趋势， GOT和GPT酶活均在盐度24‰时达到最大， AKP酶活盐度30‰达到最大。由此可见，盐度突变时幼体的盐度存活范围为21‰~30‰，最适盐度为24‰~27‰；盐度渐变时幼体的盐度存活范围为18‰~33‰，最适盐度为24‰~27‰。由此可见，盐度渐变有利幼体适盐范围拓宽、存活率提高。
위탐구염도대호반오적(Sepia pharaonis)유체생장화매활적영향，채용돌변화점변단인자시험방법연구료불동염도(18‰、21‰、24‰、27‰、30‰、33‰)조건대기존활솔、특정생장솔、증중솔、간체지수화초양화물기화매(SOD)、감성린산매(AKP)、곡병전안매(GPT)、곡초전안매(GOT)등간장대사매매활적영향。결과표명：경21d배양，염도돌변대유체존활솔화행위유현저영향(p<0.05)，염도24‰조유체존활솔최고(77.78%±6.94%)，염도27‰조특정생장솔(7.29%×d-1±0.27%×d-1)화증중솔(198.42%±12.36%)최대，간체비수착염도적상승정현상승추세，이염도30‰최고；염도점변대유체존활솔、특정생장솔、증중솔、간체비화매활영향현저(p<0.05)。염도24‰조존활솔(87.78%±1.92%)최고，염도27‰시특정생장솔(6.88%×d-1±0.13%×d-1)화증중솔(180.96%±5.13%)최대，간체비재염도30‰시체도최대。SOD매활수착염도적상승정현선하강후상승적추세，염도27‰최저，이AKP、GPT화GOT매활정현선상승후하강적추세， GOT화GPT매활균재염도24‰시체도최대， AKP매활염도30‰체도최대。유차가견，염도돌변시유체적염도존활범위위21‰~30‰，최괄염도위24‰~27‰；염도점변시유체적염도존활범위위18‰~33‰，최괄염도위24‰~27‰。유차가견，염도점변유리유체괄염범위탁관、존활솔제고。
To investigate salinity effects on growth and enzyme activity of juvenileSepia pharaonis, manipulative laboratory experiments were conducted. These experiments revealed the effects of abrupt and gradual changes of salinity on survival rate, specific growth rate, weight gain rate, hepatosomatic index, and enzyme activity (superoxide dismutase, alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic-pyruvic transaminase). The results showed that abrupt salinity change had significant effects on larval survival rate and behavior after cultivation of 21 days (p<0.05). Optimum survival rates of juvenile cuttlefish were obtained when cultivated at salinity of 24‰ and 27‰ (75.56%±6.94%~77.78%±6.94%). Specific growth rate and weight gain rate were the highest at the salinity of 27‰. The hepatosomatic index increased with salinity increase, and achieved the highest value at the salinity of 30‰. Gradual change of salinity also had significant influence on survival rate, specific growth rate, weight gain rate, hepatosomatic index, and enzyme activity (p<0.05). Optimum survival rate (87.78%±1.92%) was obtained at the salinity of 24‰. Specific growth rate (6.88%×d-1±0.13%×d-1) and weight gain rate (180.96%±5.13%) were the highest at the salinity of 27‰, whilst the highest hepatosomatic index was observed at salinity of 30‰. SOD enzyme activity declined and then increased following the rising of salinity from 18‰ to 33‰, with the lowest value at salinity of 27‰. GOT, GPT and AKP enzyme increased and then decreased; GOT and GPT enzyme activity were the highest when the salinity was 24‰, whereas AKP enzyme reached the maximum value at salinity of 30‰. Therefore, the suitable and optimal salinity values were 21‰~30‰ and 24‰~27‰, respectively, under abrupt salinity change. Suitable and optimal salinity values were 18‰~33‰ and 24‰~27‰, respectively, under gradual salinity change. We concluded that juvenile cuttlefish can live under a wider range of salinity and that we can improve its survival rate with very gradual salinity change.