CAJ | 학술논문

以改性酵母细胞为稳定剂和还原剂，在氢氧化钠存在下通过原位还原氯金酸绿色合成了荧光金纳米粒（AuNPs），并对其进行了 X-射线衍射和紫外光谱表征。基于 Cu2＋对 AuNPs 的荧光猝灭作用，建立了一种快速简便地检测食品中微量 Cu2＋的方法。结果表明，Cu2＋浓度在0.001～0.2μg·mL－1范围内，AuNPs 的荧光猝灭程度与 Cu2＋浓度间呈良好的线性关系。方法的准确度和精密度满足食品分析的要求，已成功用于大米和豆干中微量 Cu2＋的测定。
이개성효모세포위은정제화환원제，재경양화납존재하통과원위환원록금산록색합성료형광금납미립（AuNPs），병대기진행료 X-사선연사화자외광보표정。기우 Cu2＋대 AuNPs 적형광졸멸작용，건립료일충쾌속간편지검측식품중미량 Cu2＋적방법。결과표명，Cu2＋농도재0.001～0.2μg·mL－1범위내，AuNPs 적형광졸멸정도여 Cu2＋농도간정량호적선성관계。방법적준학도화정밀도만족식품분석적요구，이성공용우대미화두간중미량 Cu2＋적측정。
The fluorescent gold nanoparticles(AuNPs)were green synthesized by reduction of chloroauric acid with modified yeast cells as stabilizer and reducing agent in the presence of sodium hydroxide.AuNPs were confirmed by X-ray diffraction and UV-visible spectroscopy.A simple and rapid detection method of Cu2+ in food products was developed based on the fluorescence quenching of the as-prepared AuNPs induced by Cu2+. Results indicated that,the quenched fluorescence of AuNPs was linearly proportional to the concentration of Cu2+ in the range from 0.001 μg·mL-1 to 0.2 μg·mL-1 ,the accuracy and precision of the developed method met the requirements of food analysis,and had been successfully applied to the detection of Cu2+ in rice and dried tofu.