化学与生物工程
化學與生物工程
화학여생물공정
Chemistry & Bioengineering
2015年
11期
31-33
,共3页
提取方法%箬叶%总黄酮%抑菌活性
提取方法%箬葉%總黃酮%抑菌活性
제취방법%약협%총황동%억균활성
extraction method%indocalamus leaves%total flavonoids%antibacterial activity
通过抑菌圈直径及最低抑菌浓度的测定，分别考察了乙醇浸渍法、热回流法、微波法、超声波法提取的箬叶总黄酮对细菌（金黄色葡萄球菌、大肠杆菌）、真菌（白色念珠菌、酿酒酵母菌、青霉菌）的抑菌效果。结果表明，箬叶总黄酮对细菌的抑制作用大于对真菌的抑制作用；且提取方法对箬叶总黄酮的抑菌能力有影响，不同提取方法提取的箬叶总黄酮抑菌效果为：超声波法＞热回流法＞乙醇浸渍法＞微波法。超声波法比较适合于箬叶总黄酮的提取。
通過抑菌圈直徑及最低抑菌濃度的測定，分彆攷察瞭乙醇浸漬法、熱迴流法、微波法、超聲波法提取的箬葉總黃酮對細菌（金黃色葡萄毬菌、大腸桿菌）、真菌（白色唸珠菌、釀酒酵母菌、青黴菌）的抑菌效果。結果錶明，箬葉總黃酮對細菌的抑製作用大于對真菌的抑製作用；且提取方法對箬葉總黃酮的抑菌能力有影響，不同提取方法提取的箬葉總黃酮抑菌效果為：超聲波法＞熱迴流法＞乙醇浸漬法＞微波法。超聲波法比較適閤于箬葉總黃酮的提取。
통과억균권직경급최저억균농도적측정，분별고찰료을순침지법、열회류법、미파법、초성파법제취적약협총황동대세균（금황색포도구균、대장간균）、진균（백색념주균、양주효모균、청매균）적억균효과。결과표명，약협총황동대세균적억제작용대우대진균적억제작용；차제취방법대약협총황동적억균능력유영향，불동제취방법제취적약협총황동억균효과위：초성파법＞열회류법＞을순침지법＞미파법。초성파법비교괄합우약협총황동적제취。
Through determination of inhibition zone diameter and minimal inhibitory concentration,the anti-bacterial activities of total flavonoids from indocalamus leaves by different extraction methods of ethanol immer-sion method,hot reflux method,microwave method,and ultrasonic method were studied.Results showed that, total flavonoids from indocalamus leaves had better antibacterial activities on Escherichia coli and Staphylococ-cus aureus ,but had less activities on Moniliaalbican,Saccharomyces cerevisiae and Penicillium.The activities were affected by extraction methods,the order of antibacterial effect was as follows:ultrasonic method >hot re-flux method>ethanol immersion method>microwave method.Ultrasonic method is suitable for extracting total flavonoids from indocalamus leaves.
