CAJ | 학술논문

以1，3‐交替二乙酯硫杂杯[4]芳烃和7‐羟基香豆素为原料，分步取代杯芳烃下沿，合成得到1，3‐交替香豆素‐硫杂杯[4]芳烃荧光探针1。在DMSO／H2 O （φ，3／7，pH 7）溶液中，探针1发射的强荧光被 Fe3＋选择性猝灭；F e3＋使探针1的紫外吸收显著增强。相同条件下其他常见金属离子的加入对探针1的荧光及吸收光谱无影响，探针在水溶液介质中仅对Fe3＋高选择性识别，荧光和吸收光谱法的检测限均达10－8 mol?L －1。光谱滴定、等温滴定量热及质谱法均测出探针1与Fe3＋形成1∶1配合物，作用常数达105 L?mol－1。从摩尔结合自由能和结合熵分别表明配合作用为自发过程。同时，在DMSO／H2 O （φ，1／9，Tris‐HCl ，pH 7，0.1 mol?L －1 NaCl）介质中，探针1能选择性猝灭识别牛血红蛋白（BHb），线性范围在0.2～3.0μg?mL －1，检测限达0.12μg?mL －1。为一种选择性识别Fe3＋和BHb的杯芳烃荧光探针。
이1，3‐교체이을지류잡배[4]방경화7‐간기향두소위원료，분보취대배방경하연，합성득도1，3‐교체향두소‐류잡배[4]방경형광탐침1。재DMSO／H2 O （φ，3／7，pH 7）용액중，탐침1발사적강형광피 Fe3＋선택성졸멸；F e3＋사탐침1적자외흡수현저증강。상동조건하기타상견금속리자적가입대탐침1적형광급흡수광보무영향，탐침재수용액개질중부대Fe3＋고선택성식별，형광화흡수광보법적검측한균체10－8 mol?L －1。광보적정、등온적정량열급질보법균측출탐침1여Fe3＋형성1∶1배합물，작용상수체105 L?mol－1。종마이결합자유능화결합적분별표명배합작용위자발과정。동시，재DMSO／H2 O （φ，1／9，Tris‐HCl ，pH 7，0.1 mol?L －1 NaCl）개질중，탐침1능선택성졸멸식별우혈홍단백（BHb），선성범위재0.2～3.0μg?mL －1，검측한체0.12μg?mL －1。위일충선택성식별Fe3＋화BHb적배방경형광탐침。
1 ,3‐alternate coumarin substituted thiacalix [4]arene fluorescent probe 1 was synthesised from 1 ,3‐alternate diethyl thiacalix[4]arene and 7‐hydroxycoumarin by‐step reactions .In DMSO/H2O (φ,3/7 ,pH 7) solution ,the strong fluorescence emission and UV absorption of probe 1 can be selectively quenched or significantly enhanced by Fe3+ ion .The probe 1 showed high Fe3+ selective fluorescence quenching or absorption enhancement over commonly coexistent metal ions in neutral aqueous media ,and the limit of detections were obtained as low as 10-8 mol?L -1 of Fe3+ by fluorescence and absorption spectrometry . Spectral titration ,isothermal titration calorimetry and mass spectrometry were showed that probe and Fe3+ form 1∶1 comple‐xes ,the constant up to 105 L?mol-1 and coordinate process was spontaneous by the mole binding free energy and entropy of probe with Fe3+ .In addition ,the probe can identification bovine hemoglobin (BHb) over other proteins through quenched its fluorescenceinDMSO/H2O(φ,1/9,Tris‐HCl,pH7,0.1mol?L -1NaCl)media.Thelimitofdetectionwasobtainedas0.12μg?mL -1 of BHb ,as well as a linearity of 0.2~3.0μg?mL -1 ,indicating the probe of high sensitivity and quantitation range . It can be used as a selective recognition Fe3+ and BHb of thiacalix[4] arene fluorescent probe .