CAJ | 학술논문

基于量子点和超顺磁纳米颗粒分别构建了沙门氏菌的荧光免疫和磁驰豫时间（magnetic resonance sw itch ，M RS ）免疫传感的分析方法，并应用于水样中沙门氏菌的检测。在荧光免疫分析方法中，利用偶联有沙门氏菌捕获抗体的磁探针对样品中沙门氏菌进行富集，然后加入偶联有沙门氏菌检测抗体的量子点荧光探针，基于双抗夹心的模式对水样中沙门氏菌的含量进行了测定。量子点的荧光强度和样品中沙门氏菌的浓度呈正相关，检出限是102 cf u?m L‐1，检测时间为2 h。在M RS免疫反应过程中，偶联有沙门氏菌捕获抗体的超顺纳米磁珠会特异性地结合沙门氏菌，导致超顺纳米磁珠由原来的分散状态转变为聚集状态，从而引起其相邻水分子的质子横向弛豫时间（spin‐spin relaxation time ， T2）的改变，通过测定 T2的改变量ΔT2实现水样中沙门氏菌的检测。结果表明MRS传感器检测沙门氏菌的检测限是103 cfu?mL‐1，检测时间是0.5 h。比较了这两种方法在检测沙门氏菌方面的优劣及应用潜力。
기우양자점화초순자납미과립분별구건료사문씨균적형광면역화자치예시간（magnetic resonance sw itch ，M RS ）면역전감적분석방법，병응용우수양중사문씨균적검측。재형광면역분석방법중，이용우련유사문씨균포획항체적자탐침대양품중사문씨균진행부집，연후가입우련유사문씨균검측항체적양자점형광탐침，기우쌍항협심적모식대수양중사문씨균적함량진행료측정。양자점적형광강도화양품중사문씨균적농도정정상관，검출한시102 cf u?m L‐1，검측시간위2 h。재M RS면역반응과정중，우련유사문씨균포획항체적초순납미자주회특이성지결합사문씨균，도치초순납미자주유원래적분산상태전변위취집상태，종이인기기상린수분자적질자횡향이예시간（spin‐spin relaxation time ， T2）적개변，통과측정 T2적개변량ΔT2실현수양중사문씨균적검측。결과표명MRS전감기검측사문씨균적검측한시103 cfu?mL‐1，검측시간시0.5 h。비교료저량충방법재검측사문씨균방면적우렬급응용잠력。
Fluoroimmunoassay based on quantum dots(QDs) and magnetic relaxation switch (MRS) immunoassay based on su‐perparamagnetic nanoparticles(SMN) were constructed to detect Salmonella enterica(S .enterica) in water samples .In fluoro‐immunoassay ,magnetic beads was conjugated with S .enterica capture antibody (MB‐Ab2) to enrich S .enterica from sample solution ,then the QDs was conjugated with the S .enterica detection antibody (QDs‐Ab1) to detect S .enterica based on sand‐wich immunoassay format .And the fluorescence intensity is positive related to the bacteria concentration of the sample .Results showed that the limit of detection (LOD) of this method was 102 cfu?mL -1 and analysis time was 2 h .In MRS assay ,magnetic nanoparticle‐antibody conjugate (MN‐Ab1) can switch their dispersed and aggregated state in the presence of the target .This state of change can modulate the spin‐spin relaxation time (T2 ) of the neighboring water molecule .The change in T2 (ΔT2 ) posi‐tively correlates with the amount of the target in the sample .Thus ,ΔT2 can be used as a detection signal in MRS immunosen‐sors .Results showed that LOD of MRS sensor for S .enterica was 103 cfu?mL -1 and analysis time was 0.5 h .Two methods were compared in terms of advantages and disadvantages in detecting S .enterica .