CAJ | 학술논문

碱性橙Ⅱ是一种非食用物质，可能被非法用于食品染色。本文以十六烷基三甲基溴化铵（CTAB）为表面活性剂，采用化学合成法制备了均匀的纳米金表面增强拉曼散射活性基底，建立了灵敏快速检测碱性橙Ⅱ的拉曼光谱检测方法。运用多元变量分析方法验证各浓度之间存在显著性差异，选取碱性橙Ⅱ在1594 cm －1处的拉曼特征峰，单变量分析表明拉曼散射强度与碱性橙Ⅱ浓度的对数呈线性相关，线性范围0.001 mmol?L －1～0.5 mmol?L －1，相关系数 r＝0.9902。将本法应用于腐竹中碱性橙Ⅱ的测定，在添加浓度为50和500μg?g －1时，添加回收率为82.4％～116.9％，相对标准偏差为3.8％～4.0％。与常规化学分析技术相比，本法具有无损、快速、环保、消耗化学试剂少、所需样品量少等特点，适于对大规模样品进行筛查。
감성등Ⅱ시일충비식용물질，가능피비법용우식품염색。본문이십륙완기삼갑기추화안（CTAB）위표면활성제，채용화학합성법제비료균균적납미금표면증강랍만산사활성기저，건립료령민쾌속검측감성등Ⅱ적랍만광보검측방법。운용다원변량분석방법험증각농도지간존재현저성차이，선취감성등Ⅱ재1594 cm －1처적랍만특정봉，단변량분석표명랍만산사강도여감성등Ⅱ농도적대수정선성상관，선성범위0.001 mmol?L －1～0.5 mmol?L －1，상관계수 r＝0.9902。장본법응용우부죽중감성등Ⅱ적측정，재첨가농도위50화500μg?g －1시，첨가회수솔위82.4％～116.9％，상대표준편차위3.8％～4.0％。여상규화학분석기술상비，본법구유무손、쾌속、배보、소모화학시제소、소수양품량소등특점，괄우대대규모양품진행사사。
Chrysoidin is a kind of banned food dye ,and it has been illegally used for coloring food .A rapid detection and quanti‐fication method is developed and applied in analysis chrysoidin in yuba .Gold nanoparticles are synthesized by using hexadecyl tri‐methyl ammonium bromide (CTAB) as the bifunctional ligand to link the solid substrate and the AuNPs .The laser wavelength used for quantitative is 1 594 cm -1 .Significant differences between different concentrations of chrysoidin are verified by multiple variable analysis .A relationship between the logarithm of the concentrations and the intensity of laser is proved using univariate analysis method .The calibration curves showed good linearity in the range of 0.001~0.5 mmol? L -1 with correlation coeffi‐cients r=0.995 .The method is successfully applied to the determination of chrysoidin in yuba .The average recoveries of the drugs spiked at 50 and 500 μg?g -1 levels are 82.4% and 116.9% ,and the relative standard deviations (RSD) are 3.8% and 4.0% .The method is simple ,rapid ,sensitive and accurate in the determination of chrysoidin .