CAJ | 학술논문

从石油污染盐渍土壤中筛选出一株对液蜡乳化效果明显、对原油降解率达56.8％的菌株，命名为 BZ-L。经生理生化和16S rRNA 序列分析，初步鉴定该菌株属于沙雷氏菌属。以海藻酸钠和活性炭为包埋剂，对该菌株进行固定化研究。结果表明，当活性炭含量为0.8％时，固定化微球的破损率最低、渗透性最好；在接种量为35.0 g·L－1、NaCl 浓度为6.0％时，固定化微球对原油的降解率可达61.7％；菌株 BZ-L 的固定化微球对原油的降解率明显高于游离菌，且比游离菌的耐盐性能更强，可用于石油污染盐渍土壤的生物修复。
종석유오염염지토양중사선출일주대액사유화효과명현、대원유강해솔체56.8％적균주，명명위 BZ-L。경생리생화화16S rRNA 서렬분석，초보감정해균주속우사뢰씨균속。이해조산납화활성탄위포매제，대해균주진행고정화연구。결과표명，당활성탄함량위0.8％시，고정화미구적파손솔최저、삼투성최호；재접충량위35.0 g·L－1、NaCl 농도위6.0％시，고정화미구대원유적강해솔가체61.7％；균주 BZ-L 적고정화미구대원유적강해솔명현고우유리균，차비유리균적내염성능경강，가용우석유오염염지토양적생물수복。
A petroleum-degrading strain named as BZ-L was isolated from the petroleum contaminated saline soil,which could emulsify liquid paraffin well and degradation rate of petroleum was 56.8%.The strain was be-longed to Serratia marcescens ,according to physical and biochemical characteristics and 16S rRNA sequence a-nalysis.Sodium alginate and activated carbon were employed as embedding agents to study the immobilization of the strain.Results showed that damage rate of the immobilized microspheres were the lowest and the permeabil-ity was the best when content of activated carbon was 0.8%.The degradation rate of petroleum by the immobi-lized microspheres was 61.7%,with the inoculation amount of 35.0 g · L-1 and the NaCl concentration of 6.0%.The degradation rate of petroleum by the immobilized microspheres was higher than that of the free strain,and they were more halotolerant.Strain BZ-L and the immobilized microspheres could be used in the bioremediation of the petroleum contaminated saline soil.