CAJ | 학술논문

分别将量子点和超顺纳米磁珠作为荧光探针和磁信号探针应用于免疫反应中，构建了检测莱克多巴胺的荧光免疫和磁免疫层析的分析方法，并成功应用于尿液中莱克多巴胺的检测。两种方法均基于免疫竞争模式，在荧光免疫分析方法中，量子点偶联上识别莱克多巴胺的抗体，样品中莱克多巴胺和包被在ELISA板上莱克多巴胺的完全抗原竞争结合量子点，样品中莱克多巴胺的浓度越高，ELISA板上吸附的量子点越少，所测荧光强度值越低，该方法的检出限为1ng?mL －1，检测时间为4h。在磁免疫层析方法中，检测线上特异性捕获的纳米磁珠颜色的深浅和尿液中莱克多巴胺浓度成反比例关系，即莱克多巴胺的浓度越高，检测线的颜色越浅，该方法的定性检出限为10 ng?mL －1，检测时间为15 min。两种方法各有优缺点，基于量子点的荧光免疫分析法在痕量检测和定量分析方面具有优势，而磁免疫层析法更适合于现场快速检测。
분별장양자점화초순납미자주작위형광탐침화자신호탐침응용우면역반응중，구건료검측래극다파알적형광면역화자면역층석적분석방법，병성공응용우뇨액중래극다파알적검측。량충방법균기우면역경쟁모식，재형광면역분석방법중，양자점우련상식별래극다파알적항체，양품중래극다파알화포피재ELISA판상래극다파알적완전항원경쟁결합양자점，양품중래극다파알적농도월고，ELISA판상흡부적양자점월소，소측형광강도치월저，해방법적검출한위1ng?mL －1，검측시간위4h。재자면역층석방법중，검측선상특이성포획적납미자주안색적심천화뇨액중래극다파알농도성반비례관계，즉래극다파알적농도월고，검측선적안색월천，해방법적정성검출한위10 ng?mL －1，검측시간위15 min。량충방법각유우결점，기우양자점적형광면역분석법재흔량검측화정량분석방면구유우세，이자면역층석법경괄합우현장쾌속검측。
A fluoroimmunoassay based on quantum dots(QDs) and a lateral flow immunoassay system based on the magnetic beads (MB) were constructed to detect ractopamine (RAC) in urine samples .The monoclonal antibody (Ab1) against RAC was conjugated with QDs or MB as detector reagent ,respectively .They apply a competitive format using an immobilized RAC conju‐gate and free RAC present in samples .That is to say ,the concentration of RAC in the sample was negative related to the fluo‐rescense intensity of QDs or the color density of MB .Results showed that the limit of detection (LOD) of fluorescence immuno‐assay method is 1 ng?mL -1 and analysis time is 4 h ,while the visual LOD was 10 ng?mL -1 and analysis time was 15 min in magnetic lateral flow immunoassay system (MFLIS) .Taken into consideration of the advantages and disadvantages of the two methods ,it was suitable for the trace detection of RAC using fluoroimmunoassay while it was appropriate for point‐of‐care tesing of RAC by M FLIS .