CAJ | 학술논문

采集辽宁省大连市各大农贸批发市场鲜猪肉68份，用选择性培养基 SS琼脂培养基分离沙门氏菌，通过菌落形态及 PCR方法对分离菌株进行鉴定，利用肉汤微量稀释法药敏试验检测沙门氏菌对9大类15种抗生素的耐药性。结果表明：共获得鲜猪肉样品沙门氏菌12株，检出率为17.64%；83.33%鲜猪肉样品沙门氏菌至少耐受1种抗菌药，耐药率最高的为氟苯尼考（75.0%）、盐酸沙拉沙星（66.7%）、硫酸新霉素（66.7%）和多西环素（66.7%），其次为土霉素（58.3%）、硫酸庆大霉素（58.3%）、乙酰甲喹（58.3%），耐药率较低的有乳酸环丙沙星（50.0%）、恩诺沙星（50.0%）、盐酸环丙沙星（50.0%）、阿莫西林（16.7%）、硫酸黏菌素（16.7%）；大连市农贸批发市场鲜猪肉的沙门氏菌检出率较高，并且沙门氏菌对各类抗生素表现出了很高的耐药性，这种高耐药性也可能通过食物链传递给人类，应该引起足够重视。
채집요녕성대련시각대농무비발시장선저육68빈，용선택성배양기 SS경지배양기분리사문씨균，통과균락형태급 PCR방법대분리균주진행감정，이용육탕미량희석법약민시험검측사문씨균대9대류15충항생소적내약성。결과표명：공획득선저육양품사문씨균12주，검출솔위17.64%；83.33%선저육양품사문씨균지소내수1충항균약，내약솔최고적위불분니고（75.0%）、염산사랍사성（66.7%）、류산신매소（66.7%）화다서배소（66.7%），기차위토매소（58.3%）、류산경대매소（58.3%）、을선갑규（58.3%），내약솔교저적유유산배병사성（50.0%）、은낙사성（50.0%）、염산배병사성（50.0%）、아막서림（16.7%）、류산점균소（16.7%）；대련시농무비발시장선저육적사문씨균검출솔교고，병차사문씨균대각류항생소표현출료흔고적내약성，저충고내약성야가능통과식물련전체급인류，응해인기족구중시。
In this study, 68 fresh pork samples were col ected from several major wholesale markets in Dalian City of Liaoning Province to isolate Salmonel a using SS agar medium as selective medium. Salmonel a isolates were identified with colony morphology observation and PCR method. The resistance of Salmonel a iso-lates to 15 antibiotics was analyzed by broth microdilution susceptibility test. Accord-ing to the result, 12 Salmonel a strains were isolated from 68 fresh pork samples, indicating a detection rate of 17.64%; 83.33% of Salmonel a strains isolated from fresh pork samples could at least resist one antibiotic; Salmonel a strains exhibited the strongest resistance to florfenicol (75.0%), sarafloxacin hydrochloride (66.7%), neomycin sulfate (66.7%) and doxycycline hydrochloride (66.7%), fol owed by oxyte-tracycline (58.3%), gentamicin sulphate (58.3%) and mequindox (58.3%); Salmonel a strains exhibited relatively low resistance to ciprofloxacin lactate (50.0%), en-rofloxacin (50.0%), ciprofloxacin hydrochloride (50.0%), amoxicil in (16.7%) and col-istin sulphate (16.7%). Salmonel a in fresh pork samples from wholesale markets in Dalian Economic & Technological Development Zone exhibited a high detection rate, and the isolated Salmonel a strains showed extremely high resistance to various an-tibiotics. The strong drug-resistance of Salmonel a can also be transmitted through the food chain from the food to the people, which should attract sufficient attention.