CAJ | 학술논문

采用乙醚注入法制备胡桃醌脂质体，在单因素试验基础上，以膜材比、脂药比、注入速度为影响因素，包封率为响应值，利用Box-BehnKen试验设计原理，采用3因素3水平的响应面分析法，对胡桃醌脂质体制备工艺进行优化。胡桃醌脂质体的最佳制备条件为膜材质量比4.86：1.00，脂药质量比10.92：1.00，注入速度1.52 mL· min-1，采用最佳条件制备的胡桃醌脂质体包封率较高，验证实验测得胡桃醌脂质体的包封率为79.80％，与模型预测值相对误差为0.033％，重现性好。
채용을미주입법제비호도곤지질체，재단인소시험기출상，이막재비、지약비、주입속도위영향인소，포봉솔위향응치，이용Box-BehnKen시험설계원리，채용3인소3수평적향응면분석법，대호도곤지질체제비공예진행우화。호도곤지질체적최가제비조건위막재질량비4.86：1.00，지약질량비10.92：1.00，주입속도1.52 mL· min-1，채용최가조건제비적호도곤지질체포봉솔교고，험증실험측득호도곤지질체적포봉솔위79.80％，여모형예측치상대오차위0.033％，중현성호。
Juglone liposomes were prepared by ether injection method. One-factor-at-a-time design was used to investigate the effects of five process parameters on encapsulation efficiency. Three main process parameters that in-fluence encapsulation efficiency including lecithin/cholesterol mass ratio,lecithin/juglone mass ratio and injection speed were optimized by response surface analysis based on a three-variable and three-level Box-BehnKen experi-mental design. The optimal conditions for preparing juglone liposomes were determined as 4. 86:1. 00 of lecithin/cholesterol mass ratio,10. 92:1. 00 of lecithin/juglone mass ratio and 1. 52 mL·min-1 of injection speed. Under these conditions,the actual encapsulation efficiency of juglone liposomes was 79. 80%,which showed a relative error of 0. 033% when compared with the predictive value.