CAJ | 학술논문

为研究澳大利亚荒漠植物比拉底白刺的抗旱性，以比拉底白刺幼苗作为研究材料，在100 mmol／L甘露醇溶液模拟干旱的环境下，观察了植物形态变化，测定了植物的相对含水量（ relative water content，RWC）、过氧化物酶活性（peroxidase，POD）、丙二醛（malondiadehyde，MDA）以及游离脯氨酸（proline，Pro）含量的动态变化。结果表明，干旱处理过程中，比拉底白刺叶片从基部往上逐渐萎蔫变黄，推测其通过保护顶芽的生命力以适应干旱环境；相对含水量前期相对稳定，后逐渐下降；POD活性先缓慢升高，后快速上升；游离Pro浓度总体呈上升趋势；MDA含量先升后降。差异显著性分析表明，与对照相比，POD和游离Pro的变化趋势与对照组相比差异显著，说明二者与比拉底白刺的抗旱性具有相关性，通过减少质膜过氧化保护细胞完整性；MDA含量变化不明显，可能由于POD和游离脯氨酸的作用使得白刺细胞的膜脂系统未遭受活性氧自由基的强烈攻击。
위연구오대리아황막식물비랍저백자적항한성，이비랍저백자유묘작위연구재료，재100 mmol／L감로순용액모의간한적배경하，관찰료식물형태변화，측정료식물적상대함수량（ relative water content，RWC）、과양화물매활성（peroxidase，POD）、병이철（malondiadehyde，MDA）이급유리포안산（proline，Pro）함량적동태변화。결과표명，간한처리과정중，비랍저백자협편종기부왕상축점위언변황，추측기통과보호정아적생명력이괄응간한배경；상대함수량전기상대은정，후축점하강；POD활성선완만승고，후쾌속상승；유리Pro농도총체정상승추세；MDA함량선승후강。차이현저성분석표명，여대조상비，POD화유리Pro적변화추세여대조조상비차이현저，설명이자여비랍저백자적항한성구유상관성，통과감소질막과양화보호세포완정성；MDA함량변화불명현，가능유우POD화유리포안산적작용사득백자세포적막지계통미조수활성양자유기적강렬공격。
The drought resistance research of Australia desert plants Nitraria billardieri was focused by observing mor?phological changes and analyzing variation trends of relative water content ( RWC ) , enzymatic activity of peroxidase ( POD) , malondiadehyde ( MDA) , and proline( Pro) under drought stress simulated by 100 mmol/L mannitol solution. The results showed that the leaves turned yellow under drought stress and withered gradually from base to top, presuma?bly by protecting the vitality of buds to adapt the arid environments. The relative water content kept relatively stable at early stage and then decreased gradually. The POD activity increased slowly at first and then increased rapidly. Free Pro concentration had been rising during the treatment. MDA content increased at first and then decreased. According to the statistical analysis, both POD and free Pro increased obviously compared with control group. They reduced plasma mem?brane peroxidation and kept the cell integrity which showed drought resistance of Nitraria billardieri. MDA concentration did not change significantly, this indicated that cytoplasmic membrane system was protected and did not seriously suffer from reactive oxygen free radical attacking under drought stress probably caused by increased concentration of POD and free Pro.