CAJ | 학술논문

为了获得能有效拮抗黄栌枯萎病的大丽轮枝菌（ Verticillium dahliae）和尖孢镰刀菌（ Fusarium oxysporum）的生防菌株，采用系列稀释分离法和平板对峙法从盐碱地土壤中筛选拮抗细菌，基于形态特征、生理生化特性和16S rDNA序列分析鉴定菌株，并测定其无菌滤液和挥发性气体的抑菌活性。筛选结果表明，菌株C－2－3－2是一株能有效抑制黄栌枯萎病菌和尖孢镰刀菌的生防菌株，经鉴定其为枯草芽孢杆菌（ Bacillus subtilis）。该菌株的无菌滤液对黄栌枯萎病菌和尖孢镰刀菌均具有明显的体外抑菌作用，且能有效抑制黄栌枯萎病菌微菌核的形成。同时，其挥发性气体也能有效抑制黄栌枯萎病菌的生长。另外，研究还发现该菌株能产生铁载体和合成氨，并产生几丁质酶、纤维素酶、蛋白酶和β－1，3－葡聚糖酶。研究结果表明菌株C－2－3－2在黄栌枯萎病生物防治中具有较好的应用潜力和较高的研究价值。
위료획득능유효길항황로고위병적대려륜지균（ Verticillium dahliae）화첨포렴도균（ Fusarium oxysporum）적생방균주，채용계렬희석분리법화평판대치법종염감지토양중사선길항세균，기우형태특정、생리생화특성화16S rDNA서렬분석감정균주，병측정기무균려액화휘발성기체적억균활성。사선결과표명，균주C－2－3－2시일주능유효억제황로고위병균화첨포렴도균적생방균주，경감정기위고초아포간균（ Bacillus subtilis）。해균주적무균려액대황로고위병균화첨포렴도균균구유명현적체외억균작용，차능유효억제황로고위병균미균핵적형성。동시，기휘발성기체야능유효억제황로고위병균적생장。령외，연구환발현해균주능산생철재체화합성안，병산생궤정질매、섬유소매、단백매화β－1，3－포취당매。연구결과표명균주C－2－3－2재황로고위병생물방치중구유교호적응용잠력화교고적연구개치。
In order to isolate and characterize an antagonistic bacterial strain against Verticillium dahliae and Fusarium oxysporum, serial dilution and plate?confrontation methods were used for preliminary screening and selecting antagonistic bacteria from saline?alkali soil. Strain identification was based on morphological and physiological characteristics, and phylogenetic analysis of 16S rDNA sequence. The antagonistic effects of sterile culture filtrates and volatiles of antagonis?tic bacteria were determined. An antagonistic bacteria, named as C-2-3-2, was isolated and identified as Bacillus sub?tilis. The results indicated that sterile culture filtrate of the strain exhibited obvious in vitro inhibition against V. dahliae and F. oxysporum, and suppressed the production of microsclerotia of V. dahliae. The volatiles also effectively inhibited the mycelial growth of V. dahliae. In addition, the results indicated that C-2-3-2 could produce ammonia and sid?erophore, and also could produce cell wall degrading enzymes, such as chitinase, cellulose, protease and β-1,3-glu?canase. It indicted that strain C-2-3-2 had high research value and potentials for biological control of Verticillium wilt of Cotinus coggygria.